Song system neuroanatomy, and immediate early gene expression in a finch species with extensive male and female song

• Published in: Journal of Comparative Physiology 2024-09, Vol.210 (5), p.735-749
• Main Authors: Rose, Evangeline M., Haakenson, Chelsea M., Patel, Aliyah, Gaind, Shivika, Shank, Benjamin D., Ball, Gregory F.
• Format: Article
• Language: English
• Subjects: Animal Physiology; Animals; Biomedical and Life Sciences; Brain - metabolism; Brain - physiology; Control systems; Female; Females; Finches - genetics; Finches - physiology; Gender differences; Gene expression; Genes, Immediate-Early; Learning; Life Sciences; Male; Males; Neurosciences; Passeriformes; physiology; Progesterone; Progesterone - blood; Robust control; Sex Characteristics; Sex differences; Social behavior; Social discrimination learning; Song control nuclei; Songbirds; species; Testosterone; Testosterone - blood; vocalization; Vocalization behavior; Vocalization, Animal - physiology; Zoology
• ISSN: 0340-7594; 1432-1351; 1432-1351
• DOI: 10.1007/s00359-023-01651-9

Birdsong is a relatively well-studied behavior, both due to its importance as a model for vocal production learning and as an intriguing complex social behavior. Until the last few decades, work on birdsong focused almost exclusively on males. However, it is now widely accepted that female song not only exists, but is fairly common throughout the oscine passerines. Despite this, and the large number of researchers who have begun exploring female song in the field, researchers in the lab have been slow to adopt model species with female song. Studying female song in the lab is critical for our understanding of sex-specific factors in the physiology controlling this fascinating behavior. Additionally, as a model for vocal production learning in humans, understanding the mechanistic and neuroendocrine control of female song is clearly important. In this study, we examined the red-cheeked cordon bleu (RCCB), an Estrildid finch species with extensive female song. Specifically, we found that there were no significant sex differences in circulating levels of testosterone and progesterone, nor in song production rate. There were no significant differences in cell densities in the three nuclei of the song control system we examined. Additionally, the volume of the robust nucleus of the arcopallium was not significantly different and we report the smallest sex difference in HVC yet published in a songbird. Finally, we demonstrated similar levels of motor driven immediate early gene expression in both males and females after song production.