LncRNA BANCR promotes oral squamous cell carcinoma progression via regulating Rab1A signaling

• Published in: Journal of oral pathology & medicine 2023-09, Vol.52 (8), p.727-737
• Main Authors: Li, Weiyu, Xu, Xiaoqing, Ma, Zeyi, Shen, Peiqi, Cheng, Bin, Xia, Juan, Li, Yaoyin
• Format: Article
• Language: English
• Subjects: BANCR; Bioinformatics; Blood vessels; Cancer; Cell migration; Cell proliferation; DNA microarrays; Hybridization; Immunoprecipitation; long non‐coding RNA; Metastases; Metastasis; Molecular modelling; Motility; NF‐κB; Oral cancer; Oral carcinoma; Oral squamous cell carcinoma; Phosphorylation; Plasmids; Proteins; Rab1A; Ras protein; Signal transduction; siRNA; Squamous cell carcinoma
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/jop.13463

Background Long non‐coding RNA BRAF‐activated non‐protein coding RNA plays bidirectional roles in human cancers. However, function and molecular mechanism of BRAF‐activated non‐protein coding RNA in oral squamous cell carcinoma still need to clarify further. Methods Long non‐coding RNA microarray assay, in situ hybridization staining, clinicopathological data analysis were performed to investigate expression pattern of BRAF‐activated non‐protein coding RNA in oral squamous cell carcinoma tissue samples. Constructing ectopically expressed BRAF‐activated non‐protein coding RNA in oral squamous cell carcinoma cells via plasmids or siRNAs, then changeable abilities of proliferation and motility of these cells were observed in vitro and in vivo. RNA‐protein pulldown, RNA immunoprecipitation, and bioinformatics analyses were performed to explore potential pathways involved in BRAF‐activated non‐protein coding RNA‐based regulation of malignant progression in oral squamous cell carcinoma. Results BRAF‐activated non‐protein coding RNA was identified upregulated in oral squamous cell carcinoma tissue and correlated with nodal metastasis and clinical severity of patients. Overexpressed BRAF‐activated non‐protein coding RNA increased percentage of 5‐ethynyl‐2′‐deoxyuridine‐positive cells, viability, migration, and invasion rates of oral squamous cell carcinoma cells, while silenced BRAF‐activated non‐protein coding RNA could observe weakened effects in vitro. Xenograft tumor formed by BRAF‐activated non‐protein coding RNA‐overexpressed cells had bigger volume, faster growth rates, higher weight, and more Ki67+ cells. Pulmonary metastasis induced by BRAF‐activated non‐protein coding RNA‐silenced cells had fewer colony nodes, Ki67+ cells, and CD31+ blood vessels. Furthermore, BRAF‐activated non‐protein coding RNA was mainly localized in nucleus of oral squamous cell carcinoma cells and bound Ras‐associated binding 1A. Silencing Ras‐associated binding 1A could damage mobile ability and phosphorylation levels of nuclear factor‐κB in oral squamous cell carcinoma cells induced by overexpressing BRAF‐activated non‐protein coding RNA. Opposite trend was also observed. Conclusion Acting as a promoter in oral squamous cell carcinoma metastasis, BRAF‐activated non‐protein coding RNA promotes oral squamous cell carcinoma cells proliferation and motility by regulating the BRAF‐activated non‐protein coding RNA/Ras‐associated binding 1A complex, which activates nuclear factor‐κB sig