Biodegradation of phenanthrene by piezotolerant Bacillus subtilis EB1 and genomic insights for bioremediation

A marine strain B. subtilis EB1, isolated from Equator water, showed excellent degradation towards a wide range of hydrocarbons. Degradation studies revealed dense growth with 93 % and 83 % removal of phenanthrene within 72 h at 0.1 and 20 MPa, respectively. The identification of phenanthrene degrad...

Full description

Saved in:
Bibliographic Details
Published in:Marine pollution bulletin 2023-09, Vol.194 (Pt B), p.115151-115151, Article 115151
Main Authors: Ganesh Kumar, A., Manisha, D., Nivedha Rajan, N., Sujitha, K., Magesh Peter, D., Kirubagaran, R., Dharani, G.
Format: Article
Language:English
Subjects:
Biodegradation
Genome analysis
Hydrocarbonclastic bacteria
Phenanthrene
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A marine strain B. subtilis EB1, isolated from Equator water, showed excellent degradation towards a wide range of hydrocarbons. Degradation studies revealed dense growth with 93 % and 83 % removal of phenanthrene within 72 h at 0.1 and 20 MPa, respectively. The identification of phenanthrene degradation metabolites by GC–MS combined with its whole genome analysis provided the pathway involved in the degradation process. Whole genome sequencing indicated a genome size of 3,983,989 bp with 4331 annotated genes. The genome provided the genetic compartments, which includes monooxygenase, dioxygenase, dehydrogenase, biosurfactant synthesis catabolic genes for the biodegradation of aromatic compounds. Detailed COG and KEGG pathway analysis confirmed the genes involved in the oxygenation reaction of hydrocarbons, piezotolerance, siderophores, chemotaxis and transporter systems which were specific to adaptation for survival in extreme marine habitat. The results of this study will be a key to design an optimal bioremediation strategy for oil contaminated extreme marine environment. [Display omitted] •Marine Bacillus subtilis EB1 showed higher degradation efficiency at both atmospheric and high pressure conditions.•GC-MS analysis confirmed that degradation of phenanthrene followed phthalic acid pathway.•Whole genome analysis revealed the presence of key genes involved in catabolic and adaptation mechanisms.•First report on piezotolerant hydrocarbonoclastic B. subtilis to address the aromatic hydrocarbon pollution in extreme marine environment.
ISSN:0025-326X
1879-3363
DOI:10.1016/j.marpolbul.2023.115151