Artificial neural network (ANN)-based algorithms for high light stress phenotyping of tomato genotypes using chlorophyll fluorescence features

• Published in: Plant physiology and biochemistry 2023-08, Vol.201, p.107893-107893, Article 107893
• Main Authors: Shomali, Aida, Aliniaeifard, Sasan, Bakhtiarizadeh, Mohammad Reza, Lotfi, Mahmoud, Mohammadian, Mohammad, Vafaei Sadi, Mohammad Sadegh, Rastogi, Anshu
• Format: Article
• Language: English
• Subjects: Artificial neural network; Bruta; Feature selection; Light stress; OJIP; Photosynthesis; RFE; SVM
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2023.107893

High light (HL) is a common environmental stress directly imposes photoinhibition on the photosynthesis apparatus. Breeding plants for tolerance against HL is therefore highly demanded. Chlorophyll fluorescence (ChlF) is a sensitive indicator of stress in plants and can be evaluated using OJIP transients. In this study, we compared the ChlF features of plants exposed to HL (1200 μmol m−2 s−1) with that of control plants (300 μmol m−2 s−1). To extract the most reliable ChlF features for discrimination between HL-stressed and non-stressed plants, we applied three artificial neural network (ANN)-based algorithms, namely, Boruta, Support Vector Machine (SVM), and Recursive Feature Elimination (RFE). Feature selection algorithms identified multiple features but only two features, namely the maximal quantum yield of PSII photochemistry (FV/FM) and quantum yield of energy dissipation (ɸD0), remained consistent across all genotypes in control conditions, while exhibited variation in HL. Therefore, considered reliable features for HL stress screening. The selected features were then used for screening 14 tomato genotypes for HL. Genotypes were categorized into three groups, tolerant, semi-tolerant, and sensitive genotypes. Foliar hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents were measured as independent proxies for benchmarking selected features. Tolerant genotypes were attributed with the lowest change in H2O2 and MDA contents, while the sensitive genotypes displayed the highest magnitude of increase in H2O2 and MDA by HL treatment compared to the control. Finally, a FV/FM higher than 0.77 and ɸD0 lower than 0.24 indicates a healthy electron transfer chain (ETC) when tomato plants are exposed to HL. •FV/FM and ՓD0 are the best features for phenotyping tomato genotypes for HL stress.•Order of ANN-selected features matches the order of light-affected ETC components.•ANN identifies HL-stress indicators but not all are the best marker for screening.