The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma

The role of methylation of 9 miRNA genes in the pathogenesis of metastatic clear cell renal cell carcinoma was determined by quantitative methylation-specific PCR (MS-PCR). For 5 genes ( MIR125B-1 , MIR137 , MIR193A , MIR34B/C , and MIR375 ), a significant correlation of high methylation level with...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2023-06, Vol.175 (2), p.249-253
Main Authors: Ivanova, N. A., Burdennyi, A. M., Lukina, S. S., Filippova, E. A., Pronina, I. V., Karpukhin, A. V., Matveev, V. B., Kazubskaya, T. P., Loginov, V. I., Braga, E. A., Kushlinskii, N. E.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cancer
Carcinoma, Renal cell
Cell Biology
Clear cell-type renal cell carcinoma
Development and progression
DNA methylation
Genes
Genetic aspects
Internal Medicine
Kidney cancer
Laboratory Medicine
Lymph nodes
Metastases
Metastasis
Methylation
MicroRNA
MicroRNAs
miRNA
Oncology
Pathogenesis
Pathology
Statistical analysis
Tumors
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The role of methylation of 9 miRNA genes in the pathogenesis of metastatic clear cell renal cell carcinoma was determined by quantitative methylation-specific PCR (MS-PCR). For 5 genes ( MIR125B-1 , MIR137 , MIR193A , MIR34B/C , and MIR375 ), a significant correlation of high methylation level with late (III-IV) stages, large size (T3+T4) of the tumor, and metastasis to lymph nodes and/or distant organs was revealed. For another group of genes ( MIR125B-1 , MIR1258 , MIR193A , MIR34B/C , and MIR375 ), a statistically significant correlation of high methylation level with loss of differentiation in the tumor (G3-G4) was found, and the opposite pattern was found for MIR203A . A total of 7 microRNA genes ( MIR125B-1 , MIR1258 , MIR137 , MIR193A , MIR203A , MIR34B/C , and MIR375 ) were identified, the methylation of which is associated with the progression of metastatic clear cell renal cell carcinoma. For 6 of them (except MIR34B/C ) these data were obtained for the first time. Thus, new factors of the development and progression of clear cell renal cell carcinoma were identified as potential biomarkers for the early diagnosis and prognosis of metastatic clear cell renal cell carcinoma.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-023-05844-9