Screening differential circular RNA expression profiles and the potential role of hsa_circ_0085465 in liver cancer

Aims: This study aimed to screen the circular RNAs (circRNAs) that are differentially expressed between liver cancer and paired paracarcinoma tissues and then elucidate their role in cancer progression. Materials and Methods: High-throughput sequencing of cancer and paired paracarcinoma tissues was...

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Bibliographic Details
Published in:Journal of cancer research and therapeutics 2023-07, Vol.19 (3), p.548-555
Main Authors: Zhou, Churen, Zhu, Duo, Zhou, Sibin, Wang, Haofan, Huang, Mingsheng
Format: Article
Language:English
Subjects:
Analysis
Diagnosis
Gene expression
Genetic aspects
Genomics
Health aspects
Health savings accounts
Liver cancer
Methods
Prognosis
RNA
RNA sequencing
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Summary:Aims: This study aimed to screen the circular RNAs (circRNAs) that are differentially expressed between liver cancer and paired paracarcinoma tissues and then elucidate their role in cancer progression. Materials and Methods: High-throughput sequencing of cancer and paired paracarcinoma tissues was followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the parental genes of the differentially expressed circRNAs, which were also verified via real-time quantitative polymerase chain reaction analysis of the tissues. In addition, the function of selected circRNAs was determined using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)-2H-tetrazolium (MTS) and transwell assays. Results: Total 218 and 121 circRNAs were differentially upregulated and downregulated, respectively; these were mainly enriched with GO and KEGG terms related to biological functions. From five representatives of the differentially expressed circRNAs, we selected hsa_circ_0085465 for further analysis, discovering that its overexpression promoted the proliferation, migration, and invasion of 97 L cells. Conclusion: Taken together, our results suggest that hsa_circ_0085465 is relevant to liver cancer progression.
ISSN:0973-1482
1998-4138
DOI:10.4103/jcrt.jcrt_1868_20