Significance of O6-methyl guanine methyltransferase promoter methylation in high grade glioma patients: optimal cutoff point, CpG locus, and genetic assay

Purpose To evaluate O6-methyl guanine methyltransferase ( MGMT ) promoter methylation status in high grade glioma patients and to identify the best cutoff point as well as the most predictive CpG loci for patients survival. Method Consecutive high grade glioma patients treated with surgical gross to...

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Bibliographic Details
Published in:Journal of neuro-oncology 2023-08, Vol.164 (1), p.171-177
Main Authors: Buyuktepe, Murat, Kaplan, Ibrahim, Bayatli, Eyup, Dogan, Haldun, Ugur, Hasan Caglar
Format: Article
Language:English
Subjects:
Apoptosis
Brain cancer
Brain research
Cell cycle
Chemoradiotherapy
Chemotherapy
CpG islands
DNA methylation
DNA repair
Genetic testing
Glioma
Medical prognosis
Medicine
Medicine & Public Health
Neurology
Neurosurgery
Next-generation sequencing
O6-methylguanine-DNA methyltransferase
Observational studies
Oncology
Patients
Polymerase chain reaction
Survival
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Summary:Purpose To evaluate O6-methyl guanine methyltransferase ( MGMT ) promoter methylation status in high grade glioma patients and to identify the best cutoff point as well as the most predictive CpG loci for patients survival. Method Consecutive high grade glioma patients treated with surgical gross total resection followed by concomitant radiochemotherapy and adjuvant chemotherapy were included in this retrospective observational study. Methylation status of MGMT promoter CpG island of resected tumor tissue were evaluated using next generation sequencing assay. The outcomes were grouped as CpG 70–78, CpG 79–83, CpG 84–87, CpG 70–87, and whole promoter. Quantitative analyses were dichotomized as methylated or unmethylated based on the cutoff points set to %10, and methylation was further graded as
ISSN:0167-594X
1573-7373
DOI:10.1007/s11060-023-04397-1