Spatial and Sexual Divergence of Gut Bacterial Communities in Field Cricket Teleogryllus occipitalis (Orthoptera: Gryllidae)

• Published in: Microbial ecology 2023-11, Vol.86 (4), p.2627-2641
• Main Authors: Hirata, Kazuya, Asahi, Toru, Kataoka, Kosuke
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Bacteria; Bacteria - genetics; Biomedical and Life Sciences; Carbohydrate metabolism; Carbohydrates; Communication; Compartments; Cricket Sport; Crickets; Divergence; Ecology; Egg production; Energy consumption; Female; Gastrointestinal Microbiome; Geoecology/Natural Processes; Glycolysis; Gryllidae; Industrial production; Insects; Intestinal flora; Intestinal microflora; Life Sciences; Male; Metabolism; Metagenomics; Microbial Ecology; Microbiology; Microbiota; Microorganisms; Nature Conservation; Relative abundance; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequences; Sex; Sexes; Species diversity; Species richness; Structure-function relationships; Teleogryllus occipitalis; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-023-02265-z

The insect gut is colonized by microbes that confer a myriad of beneficial services to the host, including nutritional support, immune enhancement, and even influence behavior. Insect gut microbes show dynamic changes due to the gut compartments, sex, and seasonal and geographic influences. Crickets are omnivorous hemimetabolous insects that have sex-specific roles, such as males producing chirping sounds for communication and exhibiting fighting behavior. However, limited information is available on their gut bacterial communities, hampering studies on functional compartmentalization of the gut and sex-specific roles of the gut microbiota in omnivorous insects. Here, we report a metagenomic analysis of the gut bacteriome of the field cricket Teleogryllus occipitalis using 16S rRNA V3-V4 amplicon sequencing to identify sex- and compartment-dependent influences on its diversity and function. The structure of the gut microbiota is strongly influenced by their gut compartments rather than sex. The species richness and diversity analyses revealed large difference in the bacterial communities between the gut compartments while minor differences were observed between the sexes. Analysis of relative abundance and predicted functions revealed that nitrogen- and oxygen-dependent metabolism and amino acid turnover were subjected to functional compartmentalization in the gut. Comparisons between the sexes revealed differences in the gut microbiota, reflecting efficiency in energy use, including glycolytic and carbohydrate metabolism, suggesting a possible involvement in egg production in females. This study provides insights into the gut compartment dependent and sex-specific roles of host-gut symbiont interactions in crickets and the industrial production of crickets.