Vagal cardiac control in rats with LPS-induced lung injury

• Published in: Respiratory physiology & neurobiology 2023-10, Vol.316, p.104120-104120, Article 104120
• Main Authors: Zila, I., Kolomaznik, M., Mikolka, P., Kosutova, P., Czippelova, B., Javorka, M., Calkovska, A.
• Format: Article
• Language: English
• Subjects: Acute lung injury; Autonomic nervous system; Galectin-3; Heart rate variability; Lipopolysaccharide; Rat model
• ISSN: 1569-9048; 1878-1519
• DOI: 10.1016/j.resp.2023.104120

Heart rate variability (HRV) as an index of cardiac autonomic control in acute lung injury (ALI) has been evaluated in anaesthetized rats intratracheally instilled with bacterial lipopolysaccharide (LPS) and ventilated with breathing frequency of 60/min, 40% oxygen, inspiratory time 40%, tidal volume of 6 mL/kg. ECG was recorded before and 30, 60, 120, 180 and 240 min after LPS or saline (control) administration. HRV was quantified by time and frequency-domain analysis (mean RR interval, SDRR, RMSSD and spectral power in high frequency (HF) band. Lactate in plasma, and oxidative stress, IL-1β, IL-5, IL-12p70 and IL-13 and galectin-3 in heart tissue raised in LPS-injured rats. Overall HRV magnitude (SDRR) and marker of vagal heart rate control (RMSSD), as well as frequency domain parameter, spectral power HF was increased 120 and 180 min since ALI onset. In conclusion, LPS-induced ALI is accompanied by altered vagal cardiac control mediated by autonomic nervous system, likely based on the close relationship between immune response and vagally mediated autonomic nervous activity. •Pro- and anti-inflammatory cytokines play major roles in acute inflammation in ALI/ARDS.•ANS activity and its immune-modulatory effect is evaluated using HRV analysis.•Acute lung injury is accompanied by altered vagally mediated cardiac control.