Antioxidant and drought-acclimation responses in UV-B-exposed transgenic Nicotiana tabacum displaying constitutive overproduction of H2O2

• Published in: Photochemical & photobiological sciences 2023-10, Vol.22 (10), p.2373-2387
• Main Authors: Sáenz-de la O, Diana, Morales, Luis O., Strid, Åke, Feregrino-Perez, A. Angélica, Torres-Pacheco, Irineo, Guevara-González, Ramón G.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomaterials; Chemistry; Chemistry and Materials Science; Original Papers; Physical Chemistry; Plant Sciences
• ISSN: 1474-905X; 1474-9092
• DOI: 10.1007/s43630-023-00457-7

Hydrogen peroxide (H 2 O 2 ) is an important molecule that regulates antioxidant responses that are crucial for plant stress resistance. Exposure to low levels of ultraviolet-B radiation (UV-B, 280–315 nm) can also activate antioxidant defenses and acclimation responses. However, how H 2 O 2 and UV-B interact to promote stress acclimation remains poorly understood. In this work, a transgenic model of Nicotiana tabacum cv Xanthi nc, with elevated Mn-superoxide dismutase (Mn-SOD) activity, was used to study the interaction between the constitutive overproduction of H 2 O 2 and a 14-day UV-B treatment (1.75 kJ m −2 d −1 biologically effective UV-B). Subsequently, these plants were subjected to a 7-day moderate drought treatment to evaluate the impact on drought resistance of H 2 O 2 - and UV-dependent stimulation of the plants' antioxidant system. The UV-B treatment enhanced H 2 O 2 levels and altered the antioxidant status by increasing the epidermal flavonol index, Trolox Equivalent Antioxidant Capacity, and catalase, peroxidase and phenylalanine ammonia lyase activities in the leaves. UV-B also retarded growth and suppressed acclimation responses in highly H 2 O 2 -overproducing transgenic plants. Plants not exposed to UV-B had a higher drought resistance in the form of higher relative water content of leaves. Our data associate the interaction between Mn-SOD transgene overexpression and the UV-B treatment with a stress response. Finally, we propose a hormetic biphasic drought resistance response curve as a function of leaf H 2 O 2 content in N. tabacum cv Xanthi. Graphical abstract