Co‐Delivery of Metabolic Modulators Leads to Simultaneous Lactate Metabolism Inhibition and Intracellular Acidification for Synergistic Cancer Therapy

• Published in: Advanced materials (Weinheim) 2023-11, Vol.35 (46), p.e2305512-n/a
• Main Authors: Lee, Bowon, Park, Ok Kyu, Pan, Limin, Kim, Kang, Kang, Taegyu, Kim, Hyunjoong, Lee, Nohyun, Choi, Seung Hong, Hyeon, Taeghwan
• Format: Article
• Language: English
• Subjects: Acidification; Albumins; Anticancer properties; ATP depletion; cancer treatment; combination therapy; Depletion; Effectiveness; Efflux; Evolution; Glucose; Glucose oxidase; lactate metabolism; large‐pore mesoporous silica nanoparticles; Materials science; metabolic therapy; Metabolism; Modulators; Nanoparticles; Tumors
• ISSN: 0935-9648; 1521-4095
• DOI: 10.1002/adma.202305512

Simultaneous lactate metabolism inhibition and intracellular acidification (LIIA) is a promising approach for inducing tumor regression by depleting ATP. However, given the limited efficacy of individual metabolic modulators, a combination of various modulators is required for highly efficient LIIA. Herein, a co‐delivery system that combines lactate transporter inhibitor, glucose oxidase, and O2‐evolving nanoparticles is proposed. As a vehicle, a facile room‐temperature synthetic method for large‐pore mesoporous silica nanoparticles (L‐MSNs) is developed. O2‐evolving nanoparticles are then conjugated onto L‐MSNs, followed by immobilizing the lactate transporter inhibitor and glucose oxidase inside the pores of L‐MSNs. To load the lactate transporter inhibitor, which is too small to be directly loaded into the large pores, it is encapsulated in albumin by controlling the albumin conformation before being loaded into L‐MSNs. Notably, inhibiting lactate efflux shifts the glucose consumption mechanism from lactate metabolism to glucose oxidase reaction, which eliminates glucose and produces acid. This leads to synergistic LIIA and subsequent ATP depletion in cancer cells. Consequently, L‐MSN‐based co‐delivery of modulators for LIIA shows high anticancer efficacy in several mouse tumor models without toxicity in normal tissues. This study provides new insights into co‐delivery of small‐molecule drugs, proteins, and nanoparticles for synergistic metabolic modulation in tumors. A sufficient amount of lactate transporter inhibitor (small molecule), glucose oxidase (enzyme), and O2‐evolving nanoparticles are simultaneously delivered to cancer cells using large‐pore mesoporous silica nanoparticles as a vehicle and albumin as a carrier for small molecule. This combination synergistically induces lactate metabolism inhibition and intracellular acidification, leading to severe ATP depletion and subsequent cancer cell death.