Gas plasma-induced platelet activation corresponds to reactive species profiles and lipid oxidation

Surgical-induced hemostasis is a critical step in the closure of incisions, which is frequently achieved via electrocauterization and subsequent tissue necrotization. The latter is associated with postoperative complications. Recent in vivo work suggested reactive species-producing gas plasma techno...

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Bibliographic Details
Published in:Free radical biology & medicine 2023-10, Vol.207, p.212-225
Main Authors: Striesow, Johanna, Wesche, Jan, McKitterick, Nicholas, Busch, Larissa M., von Woedtke, Thomas, Greinacher, Andreas, Bekeschus, Sander, Wende, Kristian
Format: Article
Language:English
Subjects:
Epilipidome
Hemostasis
kINPen
Non-thermal plasma
Oxidation
Thrombocytes
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Summary:Surgical-induced hemostasis is a critical step in the closure of incisions, which is frequently achieved via electrocauterization and subsequent tissue necrotization. The latter is associated with postoperative complications. Recent in vivo work suggested reactive species-producing gas plasma technology as a pro-homeostatic agent acting via platelet activation. However, it remained elusive how platelet activation is linked to lipid and protein oxidation and the reactive species compositions. A direct relation between the reactive species composition and platelet activation was revealed by assessing the production of several reactive species and by using antioxidants. In addition, platelet lipidome and proteome analysis identified significantly regulated key lipids in the platelet activation pathway, such as diacylglycerols and phosphatidylinositol as well as oxylipins like thromboxanes. Lipid oxidation products mainly derived from phosphatidylethanolamine and phosphatidylserine species were observed at modest levels. In addition, oxidative post-translational modifications were identified on key proteins of the hemostasis machinery. This study provides new insights into oxidation-induced platelet activation in general and suggests a potential role of those processes in gas plasma-mediated hemostasis in particular. [Display omitted] •Direct relation of gas plasma-derived reactive species and human platelet activation found.•Lipidomics and proteomics revealed key lipids and proteins regulated in gas plasma-activated platelets.•Lipid oxidation products and oxidative post-translational modifications identified via high-resolution mass spectrometry.
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2023.07.024