Loading…
Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis
Background Bacterial outer membrane vesicles have gained increasing attention for its antitumor effect and application in drug delivery. However, the bacterial membrane vesicles (MVs) that are secreted by Gram-positive bacteria are rarely mentioned. Bifidobacterium has a certain anti-tumor effect, b...
Saved in:
| Published in: | Molecular biology reports 2023-09, Vol.50 (9), p.7547-7556 |
|---|---|
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203 |
| container_end_page | 7556 |
| container_issue | 9 |
| container_start_page | 7547 |
| container_title | Molecular biology reports |
| container_volume | 50 |
| creator | Jiang, Yongzhu Wang, Lanxi Yang, Bangya Ma, Guanrong Chen, Zhiqi Ma, Jing Chang, Xiulin Fang, Liaoqiong Wang, Zhibiao |
| description | Background
Bacterial outer membrane vesicles have gained increasing attention for its antitumor effect and application in drug delivery. However, the bacterial membrane vesicles (MVs) that are secreted by Gram-positive bacteria are rarely mentioned.
Bifidobacterium
has a certain anti-tumor effect, but there is a certain risk when injected into human body. Here we investigated the potential of
Bifidobacterium
-derived membrane vesicles (B-MVs) as therapeutic agents to treat triple-negative breast cancer.
Methods and results
Firstly, we discovered that
B
ifidobacterium
can produce B-MVs and isolated them. In vivo, we found that B-MVs can inhibit tumor growth in mice and the mice were in good state. H&E staining displayed extensive apoptotic cells in tumor tissues. Western blotting and immunohistochemistry showed that B-MVs increased the expression of Bax, while decreased the expression of Bcl-2. These results suggested that B-MVs may induce apoptosis of tumor cells in vivo. Furthermore, to further confirm this phenomenon, we conducted experiments in vitro. Hoechst 33,258 staining assay, flow cytometry and western blotting also demonstrated B-MVs promoted cell apoptosis in vitro.
Conclusions
We speculate B-MVs may inhibit tumor growth by inducing tumor cell apoptosis in triple-negative breast cancer, which provided a new direction in the treatment of TNBC.
Graphical abstract |
| doi_str_mv | 10.1007/s11033-023-08702-z |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2843035318</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2857701734</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203</originalsourceid><addsrcrecordid>eNp9kcFuFSEUhonR2NvqC7gwJG7cUA_DMHCXtam2SRM3uibAnLmlmRmmwLRpn17qrW3iwgU5CXzn58BHyAcOxxxAfcmcgxAMmrq0goY9vCIbLpVg7Vbp12QDAjhrteQH5DDnawBouZJvyYFQ7Va3Qm_I3dcwhD466wumsE6sr-UWezrh5JKdkd5iDn7ETMN8FVwotKSwjMhm3NlSUeoS2lyot7PHRHcp3pUr6u4r368-zDta1ikm6nEcqV3iUmIO-R15M9gx4_unekR-fTv7eXrOLn98vzg9uWReKFlYq7Dj4L3ocBCu1VZte89lP7i6w_UAWy0H26N1QmsplVTQWe0077oGoQFxRD7vc5cUb1bMxUwhP45SnxbXbJr6DSCk4Lqin_5Br-Oa5jpdpaRSwJVoK9XsKZ9izgkHs6Qw2XRvOJhHLWavxVQt5o8W81CbPj5Fr27C_rnlr4cKiD2Q69G8w_Ry939ifwPKG5qe</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2857701734</pqid></control><display><type>article</type><title>Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis</title><source>Springer Nature</source><creator>Jiang, Yongzhu ; Wang, Lanxi ; Yang, Bangya ; Ma, Guanrong ; Chen, Zhiqi ; Ma, Jing ; Chang, Xiulin ; Fang, Liaoqiong ; Wang, Zhibiao</creator><creatorcontrib>Jiang, Yongzhu ; Wang, Lanxi ; Yang, Bangya ; Ma, Guanrong ; Chen, Zhiqi ; Ma, Jing ; Chang, Xiulin ; Fang, Liaoqiong ; Wang, Zhibiao</creatorcontrib><description>Background
Bacterial outer membrane vesicles have gained increasing attention for its antitumor effect and application in drug delivery. However, the bacterial membrane vesicles (MVs) that are secreted by Gram-positive bacteria are rarely mentioned.
Bifidobacterium
has a certain anti-tumor effect, but there is a certain risk when injected into human body. Here we investigated the potential of
Bifidobacterium
-derived membrane vesicles (B-MVs) as therapeutic agents to treat triple-negative breast cancer.
Methods and results
Firstly, we discovered that
B
ifidobacterium
can produce B-MVs and isolated them. In vivo, we found that B-MVs can inhibit tumor growth in mice and the mice were in good state. H&E staining displayed extensive apoptotic cells in tumor tissues. Western blotting and immunohistochemistry showed that B-MVs increased the expression of Bax, while decreased the expression of Bcl-2. These results suggested that B-MVs may induce apoptosis of tumor cells in vivo. Furthermore, to further confirm this phenomenon, we conducted experiments in vitro. Hoechst 33,258 staining assay, flow cytometry and western blotting also demonstrated B-MVs promoted cell apoptosis in vitro.
Conclusions
We speculate B-MVs may inhibit tumor growth by inducing tumor cell apoptosis in triple-negative breast cancer, which provided a new direction in the treatment of TNBC.
Graphical abstract</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-023-08702-z</identifier><identifier>PMID: 37498438</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Antitumor activity ; Apoptosis ; Bacterial vesicles ; Bcl-2 protein ; Bifidobacterium ; Biomedical and Life Sciences ; Breast cancer ; Drug delivery ; Flow cytometry ; Gram-positive bacteria ; Histology ; Immunohistochemistry ; Life Sciences ; Membrane vesicles ; Morphology ; Original Article ; Tumor cells ; Tumors ; Western blotting</subject><ispartof>Molecular biology reports, 2023-09, Vol.50 (9), p.7547-7556</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203</citedby><cites>FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203</cites><orcidid>0000-0002-0257-2577</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37498438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Yongzhu</creatorcontrib><creatorcontrib>Wang, Lanxi</creatorcontrib><creatorcontrib>Yang, Bangya</creatorcontrib><creatorcontrib>Ma, Guanrong</creatorcontrib><creatorcontrib>Chen, Zhiqi</creatorcontrib><creatorcontrib>Ma, Jing</creatorcontrib><creatorcontrib>Chang, Xiulin</creatorcontrib><creatorcontrib>Fang, Liaoqiong</creatorcontrib><creatorcontrib>Wang, Zhibiao</creatorcontrib><title>Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Bacterial outer membrane vesicles have gained increasing attention for its antitumor effect and application in drug delivery. However, the bacterial membrane vesicles (MVs) that are secreted by Gram-positive bacteria are rarely mentioned.
Bifidobacterium
has a certain anti-tumor effect, but there is a certain risk when injected into human body. Here we investigated the potential of
Bifidobacterium
-derived membrane vesicles (B-MVs) as therapeutic agents to treat triple-negative breast cancer.
Methods and results
Firstly, we discovered that
B
ifidobacterium
can produce B-MVs and isolated them. In vivo, we found that B-MVs can inhibit tumor growth in mice and the mice were in good state. H&E staining displayed extensive apoptotic cells in tumor tissues. Western blotting and immunohistochemistry showed that B-MVs increased the expression of Bax, while decreased the expression of Bcl-2. These results suggested that B-MVs may induce apoptosis of tumor cells in vivo. Furthermore, to further confirm this phenomenon, we conducted experiments in vitro. Hoechst 33,258 staining assay, flow cytometry and western blotting also demonstrated B-MVs promoted cell apoptosis in vitro.
Conclusions
We speculate B-MVs may inhibit tumor growth by inducing tumor cell apoptosis in triple-negative breast cancer, which provided a new direction in the treatment of TNBC.
Graphical abstract</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Antitumor activity</subject><subject>Apoptosis</subject><subject>Bacterial vesicles</subject><subject>Bcl-2 protein</subject><subject>Bifidobacterium</subject><subject>Biomedical and Life Sciences</subject><subject>Breast cancer</subject><subject>Drug delivery</subject><subject>Flow cytometry</subject><subject>Gram-positive bacteria</subject><subject>Histology</subject><subject>Immunohistochemistry</subject><subject>Life Sciences</subject><subject>Membrane vesicles</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Tumor cells</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kcFuFSEUhonR2NvqC7gwJG7cUA_DMHCXtam2SRM3uibAnLmlmRmmwLRpn17qrW3iwgU5CXzn58BHyAcOxxxAfcmcgxAMmrq0goY9vCIbLpVg7Vbp12QDAjhrteQH5DDnawBouZJvyYFQ7Va3Qm_I3dcwhD466wumsE6sr-UWezrh5JKdkd5iDn7ETMN8FVwotKSwjMhm3NlSUeoS2lyot7PHRHcp3pUr6u4r368-zDta1ikm6nEcqV3iUmIO-R15M9gx4_unekR-fTv7eXrOLn98vzg9uWReKFlYq7Dj4L3ocBCu1VZte89lP7i6w_UAWy0H26N1QmsplVTQWe0077oGoQFxRD7vc5cUb1bMxUwhP45SnxbXbJr6DSCk4Lqin_5Br-Oa5jpdpaRSwJVoK9XsKZ9izgkHs6Qw2XRvOJhHLWavxVQt5o8W81CbPj5Fr27C_rnlr4cKiD2Q69G8w_Ry939ifwPKG5qe</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Jiang, Yongzhu</creator><creator>Wang, Lanxi</creator><creator>Yang, Bangya</creator><creator>Ma, Guanrong</creator><creator>Chen, Zhiqi</creator><creator>Ma, Jing</creator><creator>Chang, Xiulin</creator><creator>Fang, Liaoqiong</creator><creator>Wang, Zhibiao</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0257-2577</orcidid></search><sort><creationdate>20230901</creationdate><title>Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis</title><author>Jiang, Yongzhu ; Wang, Lanxi ; Yang, Bangya ; Ma, Guanrong ; Chen, Zhiqi ; Ma, Jing ; Chang, Xiulin ; Fang, Liaoqiong ; Wang, Zhibiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Antitumor activity</topic><topic>Apoptosis</topic><topic>Bacterial vesicles</topic><topic>Bcl-2 protein</topic><topic>Bifidobacterium</topic><topic>Biomedical and Life Sciences</topic><topic>Breast cancer</topic><topic>Drug delivery</topic><topic>Flow cytometry</topic><topic>Gram-positive bacteria</topic><topic>Histology</topic><topic>Immunohistochemistry</topic><topic>Life Sciences</topic><topic>Membrane vesicles</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Tumor cells</topic><topic>Tumors</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Yongzhu</creatorcontrib><creatorcontrib>Wang, Lanxi</creatorcontrib><creatorcontrib>Yang, Bangya</creatorcontrib><creatorcontrib>Ma, Guanrong</creatorcontrib><creatorcontrib>Chen, Zhiqi</creatorcontrib><creatorcontrib>Ma, Jing</creatorcontrib><creatorcontrib>Chang, Xiulin</creatorcontrib><creatorcontrib>Fang, Liaoqiong</creatorcontrib><creatorcontrib>Wang, Zhibiao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Yongzhu</au><au>Wang, Lanxi</au><au>Yang, Bangya</au><au>Ma, Guanrong</au><au>Chen, Zhiqi</au><au>Ma, Jing</au><au>Chang, Xiulin</au><au>Fang, Liaoqiong</au><au>Wang, Zhibiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>50</volume><issue>9</issue><spage>7547</spage><epage>7556</epage><pages>7547-7556</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Bacterial outer membrane vesicles have gained increasing attention for its antitumor effect and application in drug delivery. However, the bacterial membrane vesicles (MVs) that are secreted by Gram-positive bacteria are rarely mentioned.
Bifidobacterium
has a certain anti-tumor effect, but there is a certain risk when injected into human body. Here we investigated the potential of
Bifidobacterium
-derived membrane vesicles (B-MVs) as therapeutic agents to treat triple-negative breast cancer.
Methods and results
Firstly, we discovered that
B
ifidobacterium
can produce B-MVs and isolated them. In vivo, we found that B-MVs can inhibit tumor growth in mice and the mice were in good state. H&E staining displayed extensive apoptotic cells in tumor tissues. Western blotting and immunohistochemistry showed that B-MVs increased the expression of Bax, while decreased the expression of Bcl-2. These results suggested that B-MVs may induce apoptosis of tumor cells in vivo. Furthermore, to further confirm this phenomenon, we conducted experiments in vitro. Hoechst 33,258 staining assay, flow cytometry and western blotting also demonstrated B-MVs promoted cell apoptosis in vitro.
Conclusions
We speculate B-MVs may inhibit tumor growth by inducing tumor cell apoptosis in triple-negative breast cancer, which provided a new direction in the treatment of TNBC.
Graphical abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37498438</pmid><doi>10.1007/s11033-023-08702-z</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0257-2577</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2023-09, Vol.50 (9), p.7547-7556 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2843035318 |
| source | Springer Nature |
| subjects | Animal Anatomy Animal Biochemistry Antitumor activity Apoptosis Bacterial vesicles Bcl-2 protein Bifidobacterium Biomedical and Life Sciences Breast cancer Drug delivery Flow cytometry Gram-positive bacteria Histology Immunohistochemistry Life Sciences Membrane vesicles Morphology Original Article Tumor cells Tumors Western blotting |
| title | Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A32%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bifidobacterium-derived%20membrane%20vesicles%20inhibit%20triple-negative%20breast%20cancer%20growth%20by%20inducing%20tumor%20cell%20apoptosis&rft.jtitle=Molecular%20biology%20reports&rft.au=Jiang,%20Yongzhu&rft.date=2023-09-01&rft.volume=50&rft.issue=9&rft.spage=7547&rft.epage=7556&rft.pages=7547-7556&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-023-08702-z&rft_dat=%3Cproquest_cross%3E2857701734%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c375t-47e610cc36ef3b48a79dc15dfb36e18f0985fadeab3885575706a8b81662e0203%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2857701734&rft_id=info:pmid/37498438&rfr_iscdi=true |