An MCP-based system for beta autoradiography

Describes a pre-commercial prototype autoradiography system which can be used to image a variety of radio-labelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole-body tissue slices. These samples can be imaged with high spatial resolution (/spl sim/70 microns) an...

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Bibliographic Details
Published in:IEEE transactions on nuclear science 1999-06, Vol.46 (3), p.636-638
Main Authors: Lees, J.E., Pearson, J.F., Fraser, G.W., Hales, J.M., Richards, P.G.
Format: Article
Language:English
Subjects:
Anodes
Autoradiography
Beta
Beta ray spectrometers
Biological
Biological materials
Cameras
Detectors
Dynamic range
Dynamical systems
Electrokinetics
High-resolution imaging
Lymphocytes
Microchannel
Microchannel plates
Optical resolving power
Particle detectors
Pollution measurement
Prototypes
Radioactive materials
Spatial resolution
Systems analysis
Tritium
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Description
Summary:Describes a pre-commercial prototype autoradiography system which can be used to image a variety of radio-labelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole-body tissue slices. These samples can be imaged with high spatial resolution (/spl sim/70 microns) and high sensitivity (e.g. /sup 3/H-minimum detectable activity 0.001 Bq in 20 hours) over a wide (5-6 orders of magnitude) dynamic range. Specimens are automatically presented, through a vacuum load-lock, to an imaging microchannel plate (MCP) detector operating in high (/spl sim/10/sup -6/ mbar) vacuum. We present images obtained from tritium-labelled samples and discuss the importance of the new system for a variety of biological techniques.
ISSN:0018-9499
1558-1578
DOI:10.1109/23.775591