Endophytic fungus from Handroanthus impetiginosus immobilized on electrospun nanofibrous membrane for bioremoval of bisphenol A

• Published in: World journal of microbiology & biotechnology 2023-10, Vol.39 (10), p.261-261, Article 261
• Main Authors: Conceição, João Carlos Silva, Alvarega, Augusto D., Mercante, Luiza A., Correa, Daniel S., Silva, Eliane Oliveira
• Format: Article
• Language: English
• Subjects: Adsorption; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Bioremediation; Biotechnology; Biotransformation; Bisphenol A; Cosmetics; Endocrine disruptors; Endophytes; Environmental Engineering/Biotechnology; Enzymatic activity; Fungi; Handroanthus impetiginosus; High performance liquid chromatography; Immobilization; Life Sciences; Liquid chromatography; Membranes; Metabolites; Microbiology; Pollutant removal; Pollutants; Polyacrylonitrile; Scanning electron microscopy; Toys; Water pollution; Water resources
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-023-03715-z

The current industrial and human activities scenario has accelerated the widespread use of endocrine-disrupting compounds (EDCs), which can be found in everyday products, including plastic containers, bottles, toys, cosmetics, etc., but can pose a severe risk to human health and the environment. In this regard, fungal bioremediation appears as a green and cost-effective approach to removing pollutants from water resources. Besides, immobilizing fungal cells onto nanofibrous membranes appears as an innovative strategy to improve remediation performance by allowing the adsorption and degradation to occur simultaneously. Herein, we developed a novel nanostructured bioremediation platform based on polyacrylonitrile nanofibrous membrane (PAN NFM) as supporting material for immobilizing an endophytic fungus to remove bisphenol A (BPA), a typical EDC. The endophytic strain was isolated from Handroanthus impetiginosus leaves and identified as Phanerochaete sp. H2 by molecular methods. The successful assembly of fungus onto the PAN NFM surface was confirmed by scanning electron microscopy (SEM). Compared with free fungus cells, the PAN@H2 NFM displayed a high BPA removal efficiency (above 85%) at an initial concentration of 5 ppm, suggesting synergistic removal by simultaneous adsorption and biotransformation. Moreover, the biotransformation pathway was investigated, and the chemical structures of fungal metabolites of BPA were identified by ultra-high performance liquid chromatography - high-resolution mass (UHPLC-HRMS) analysis. In general, our results suggest that by combining the advantages of enzymatic activity and nanofibrous structure, the novel platform has the potential to be applied in the bioremediation of varied EDCs or even other pollutants found in water resources.