Electrochemical immunosensor AuNPs/NG-PANI/ITO-PET for the determination of BDNF in depressed mice serum

A novel electrochemical immunosensor was developed for highly sensitive detection of brain-derived neurotrophic factor (BDNF), a well-known depression marker. The immunosensor was fabricated by modifying indium tin oxide-coated polyethylene terephthalate (ITO-PET) with N-doped graphene-polyaniline (...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2023-08, Vol.190 (8), p.330-330, Article 330
Main Authors: Wei, Hong, Sun, Bolu, Li, YuanYuan, Wang, Yanping, Chen, Yan, Guo, Min, Mo, Xiaohui, Hu, Fangdi, Du, Yongling
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antibodies
Biotin
Brain-derived neurotrophic factor
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Gold
Graphene
Immunosensors
Indium tin oxides
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Original Paper
Peroxidase
Polyanilines
Polyethylene terephthalate
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Summary:A novel electrochemical immunosensor was developed for highly sensitive detection of brain-derived neurotrophic factor (BDNF), a well-known depression marker. The immunosensor was fabricated by modifying indium tin oxide-coated polyethylene terephthalate (ITO-PET) with N-doped graphene-polyaniline (NG-PANI) and gold nanoparticles (AuNPs) to enhance the conductivity and protein loading capacity. Subsequently, BDNF was immobilized onto the electrode surface via gold-sulfur bonds, followed by the attachment of biotinylated antibody (Biotin-Ab) and horseradish peroxidase-avidin (HRP-Avidin) to create the final immunosensor (HRP-Avidin–Biotin-Ab-BDNF-AuNPs/NG-PANI/ITO-PET). The proposed immunosensor exhibited a linear range of determination (0.781–400 pg/mL) with a low limit of detection (LOD) of 0.261 pg/mL (S/N = 3) and excellent reproducibility (RSD = 1.4%) and stability (92.7%, RSD = 3.1%). Additionally, the immunosensor demonstrated good anti-interference performance and good recovery (98.1–107%). To evaluate the practical utility of the immunosensor, BDNF levels were quantified in the serum of mice with depression induced by chronic unpredictable mild stress (CUMS). The results indicated that the serum BDNF levels were significantly decreased in the depression model group compared with the control group, highlighting the potential of this immunosensor for clinical detection of BDNF in depression diagnosis and treatment. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-023-05878-w