Development of a green liquid-phase microextraction procedure using a customized device for the comprehensive determination of legacy and current pesticides in distinct types of wine samples

• Published in: Talanta (Oxford) 2024-01, Vol.266 (Pt 1), p.124914-124914, Article 124914
• Main Authors: Nascimento, Madson M., dos Anjos, Jeancarlo P., Nascimento, Melise L., Assis Felix, Caio Silva, da Rocha, Gisele O., de Andrade, Jailson B.
• Format: Article
• Language: English
• Subjects: Chemometrics; Customized devices; Gas chromatography; Green analytical chemistry; Mass spectrometry
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2023.124914

In this work, we reported the development of a novel, simple, and green liquid-phase microextraction (LPME) procedure based on the use of a customized device for the determination of 47 multiclass pesticides in red, white, and rosè wine samples by GC-MS. The main parameters that affect the LPME were optimized using multivariate statistical techniques such as centroid-simplex mixture design and Doehlert design. The optimal conditions were: 70 μL of toluene as extractor solvent; concentration of NaCl (2.7%, m v−1); pH 4; and an extraction time of 30 min, under vortex-assisted agitation (at 500 rpm). After validation, it was possible to obtain LOQ values as low as 7.63 ng L−1 and extraction recoveries ranging from 81.7% to 119% for most of the target pesticides. The application of exploratory analysis, specifically Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), provided evidence indicating contamination in the different types of wine samples, primarily by systemic fungicides. [Display omitted] •A straightforward LPME procedure was developed to simultaneously detect 47 pesticides.•The proposed procedure stands out for its low solvent and sample consumption.•Systemic fungicides were the most prevalent pesticides in the wine samples.•The AGREE metric has confirmed the greenness of the proposed procedure.