Specific knockout of Notch2 in Treg cells significantly inhibits the growth and proliferation of head and neck squamous cell carcinoma in mice

•Specific knockout of Notch2 gene in Treg cells can significantly inhibit the growth of HNSCC.•Specific knockout of Notch2 in Treg cells can significantly inhibit the function of Tregs and enhance the anti-HNSCC effect.•The tumor tissues had significant pyroptosis in Treg cell-specific Notch2-knocko...

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Bibliographic Details
Published in:International immunopharmacology 2023-10, Vol.123, p.110705-110705, Article 110705
Main Authors: Wei, Jun-Hua, Qiao, Yue-long, Xu, Shan, Zou, You, Ni, Hai-Feng, Wu, Li-Zhi, Tao, Ze-Zhang, Jiao, Wo-Er, Chen, Shi-Ming
Format: Article
Language:English
Subjects:
Antitumor immunity
Head and neck squamous cell carcinoma
Knockout mice
Notch2
Regulatory T cells
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Summary:•Specific knockout of Notch2 gene in Treg cells can significantly inhibit the growth of HNSCC.•Specific knockout of Notch2 in Treg cells can significantly inhibit the function of Tregs and enhance the anti-HNSCC effect.•The tumor tissues had significant pyroptosis in Treg cell-specific Notch2-knockout mice. To investigate the effect of Notch2 gene knockout in Treg cells on head and neck squamous cell carcinoma (HNSCC) in mice. A mouse model of HNSCC was constructed. Flow cytometry and immunofluorescence were used to examine the numbers of related immune cells and programmed cell death in tumor cells in the spleen and tumor microenvironment of mice. Western blotting was used to measure the expression of related proteins in tumor tissues. The tumor volume of regulatory T (Treg) cell-specific Notch2-knockout mice (experimental group) was significantly smaller than that of control mice (control group) (P 
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2023.110705