Effect of Chlorhexidine and Tea Tree Oil on Reducing the Number of Oral Microorganisms

• Published in: European journal of dentistry 2024-02, Vol.18 (1), p.397-400
• Main Authors: Moreno, André Luiz de Melo, de Moraes Melo Neto, Clóvis Lamartine, Coelho Goiato, Marcelo, Moreno, Nathaly Vilene de Araujo, Dos Santos, Daniela Micheline, de Lima, Cássia Cunha, Lopes Motta, Rogério Heládio, Ramacciato, Juliana Cama
• Format: Article
• Language: English
• ISSN: 1305-7456; 1305-7464
• DOI: 10.1055/s-0043-1769900

Thus, the aim of this study was to compare the effect of using two preoperative mouthwashes (0.12% chlorhexidine and 0.2% tea tree oil) on the number of colonies of oral microorganisms.  Forty participants who needed to be rehabilitated with dental implants were included in this study. They were randomly divided into two groups (chlorhexidine group and tea tree group;  = 20, each). For each group, saliva samples were collected at four different times: T0 (initially)-before using the mouthwash, T1-after 1 minute of using the mouthwash, T10-after 10 minutes of using the mouthwash, and T60-after 60 minutes of using the mouthwash. At T0 and T1, saliva samples were collected before implant placement surgery, and at T10 and T60, saliva samples were collected during surgery. In each group, one saliva sample was collected at each evaluated time point for each patient, totaling 4 saliva collections per patient. MSB agar (Mitis-Salivarius-Bacitracin) and BHI agar (Brain Heart Infusion) culture media were used in each group. Microbial colony counts were performed using a magnifying glass and recorded in CFU (colony forming units)/mL. Statistical analyses were performed using the Friedman, Mann-Whitney U and Wilcoxon tests (