Intraoperative application of optical spectroscopy in the presence of blood

A simple but effective method of spectral processing was developed to minimize or remove the effects of the presence of superficial blood on tissue optical spectra and, hence, enhance the performance of optical-spectroscopic-based in vivo tissue diagnosis and surgical guidance. This spectral-process...

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Bibliographic Details
Published in:IEEE journal of selected topics in quantum electronics 2001-11, Vol.7 (6), p.996-1003
Main Authors: Wei-Chiang Lin, Toms, S.A., Jansen, E.D., Mahadevan-Jansen, A.
Format: Article
Language:English
Subjects:
Algorithms
Attenuation
Blood
Brain modeling
Computer simulation
Diagnosis
Fluorescence
Hemoglobin
In vivo
Optical attenuators
Optical distortion
Reflectance
Reflectivity
Spectra
Spectroscopic analysis
Spectroscopy
Spectrum analysis
Stimulated emission
Surgery
Surgical implants
Tissue
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Summary:A simple but effective method of spectral processing was developed to minimize or remove the effects of the presence of superficial blood on tissue optical spectra and, hence, enhance the performance of optical-spectroscopic-based in vivo tissue diagnosis and surgical guidance. This spectral-processing algorithm was developed using the principles of absorption-induced light attenuation wherein the ratio of fluorescence intensity (F) and the hth power of diffuse reflectance intensity (Rd) at a given emission wavelength /spl lambda//sub m/ is immune to spectral distortions induced by the presence of blood on the tissue surface. Here, the exponent h is determined by the absorption coefficients of whole blood at the excitation and emission wavelengths. The theoretical basis of this spectral processing was verified using simulations and was experimentally validated. Furthermore, the optical spectra of brain tissues collected in vivo was processed using this algorithm to evaluate its impact on brain tissue differentiation using combined fluorescence and diffuse reflectance spectroscopy. Based on the simulation, as well as experimental results, it was observed that using F/Rd/sup h/ h can effectively reduce or remove spectral distortions induced by superficial blood contamination on tissue optical spectra. Thus, optical spectroscopy can also be used intraoperatively for applications such as surgical guidance of tumor resection.
ISSN:1077-260X
1558-4542
DOI:10.1109/2944.983305