Mutanase from Trichoderma harzianum inductively Produced by Mutan: Short-Term Treatment to Degrade Mature Streptococcus mutans Biofilm

This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutan s biofilms ( n  = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilm...

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Published in:Current microbiology 2023-09, Vol.80 (9), p.312-312, Article 312
Main Authors: Bem, Jéssica Silva Peixoto, Lacerda, Nayanna Gomes Silva, Polizello, Ana Cristina Morseli, Cabral, Hamilton, da Rosa-Garzon, Nathalia Gonsales, Aires, Carolina Patrícia
Format: Article
Language:English
Subjects:
Bacteria
Biofilms
Biomedical and Life Sciences
Biotechnology
Enzymes
Fungi
Life Sciences
Metabolism
Microbiology
Polysaccharides
Saccharides
Scanning electron microscopy
Sodium chloride
Streptococcus infections
Streptococcus mutans
Trichoderma harzianum
Viability
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Summary:This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutan s biofilms ( n  = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilms were analyzed by amount of matrix water-insoluble polysaccharides, bacterial viability, acidogenicity, and morphology by scanning electron microscopy (SEM). The group treated with active enzymes (AE) had a significantly lower amount of insoluble polysaccharides (893.30 ± 293.69) when compared to the negative control group (NaCl, 2192.59 ± 361.96), yet no significant difference was found when comparing to the positive control group (CHX, 436.82 ± 151.07). Also, there was no significant effect on bacteria metabolism and viability ( P -value 
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-023-03417-7