Mutanase from Trichoderma harzianum inductively Produced by Mutan: Short-Term Treatment to Degrade Mature Streptococcus mutans Biofilm

This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutan s biofilms ( n  = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilm...

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Published in:Current microbiology 2023-09, Vol.80 (9), p.312-312, Article 312
Main Authors: Bem, Jéssica Silva Peixoto, Lacerda, Nayanna Gomes Silva, Polizello, Ana Cristina Morseli, Cabral, Hamilton, da Rosa-Garzon, Nathalia Gonsales, Aires, Carolina Patrícia
Format: Article
Language:English
Subjects:
Bacteria
Biofilms
Biomedical and Life Sciences
Biotechnology
Enzymes
Fungi
Life Sciences
Metabolism
Microbiology
Polysaccharides
Saccharides
Scanning electron microscopy
Sodium chloride
Streptococcus infections
Streptococcus mutans
Trichoderma harzianum
Viability
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creator Bem, Jéssica Silva Peixoto
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description This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutan s biofilms ( n  = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilms were analyzed by amount of matrix water-insoluble polysaccharides, bacterial viability, acidogenicity, and morphology by scanning electron microscopy (SEM). The group treated with active enzymes (AE) had a significantly lower amount of insoluble polysaccharides (893.30 ± 293.69) when compared to the negative control group (NaCl, 2192.59 ± 361.96), yet no significant difference was found when comparing to the positive control group (CHX, 436.82 ± 151.07). Also, there was no significant effect on bacteria metabolism and viability ( P -value 
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subjects Bacteria
Biofilms
Biomedical and Life Sciences
Biotechnology
Enzymes
Fungi
Life Sciences
Metabolism
Microbiology
Polysaccharides
Saccharides
Scanning electron microscopy
Sodium chloride
Streptococcus infections
Streptococcus mutans
Trichoderma harzianum
Viability
title Mutanase from Trichoderma harzianum inductively Produced by Mutan: Short-Term Treatment to Degrade Mature Streptococcus mutans Biofilm
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