Ultrashort wave alleviates oxygen - glucose deprivation/reoxygenation injury via up - regulation of SPCA1 expression in N2a cells

Application of ultrashort wave (USW) to rats with cerebral ischemia and reperfusion injury could inhibit the decrease of expression of secretory pathway Ca -ATPase 1 (SPCA1), an important participant in Golgi stress, reduce the damage of Golgi apparatus and the apoptosis of neuronal cells, thereby a...

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Bibliographic Details
Published in:Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2023-05, Vol.48 (5), p.641-647
Main Authors: Tang, Jinling, Wang, Rumi, Fan, Yongmei, Zhang, Changjie, Kong, Ying
Format: Article
Language:chi ; eng
Subjects:
Animals
Apoptosis
Brain Ischemia
Calcium-Transporting ATPases - metabolism
Glucose - metabolism
Oxygen - metabolism
Rats
Reperfusion Injury - metabolism
Transcriptional Activation
Up-Regulation
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Summary:Application of ultrashort wave (USW) to rats with cerebral ischemia and reperfusion injury could inhibit the decrease of expression of secretory pathway Ca -ATPase 1 (SPCA1), an important participant in Golgi stress, reduce the damage of Golgi apparatus and the apoptosis of neuronal cells, thereby alleviating cerebral ischemia-reperfusion injury. This study aims to investigate the effect of USW on oxygen-glucose deprivation/reperfusion (OGD/R) injury and the expression of SPCA1 at the cellular level. N2a cells were randomly divided into a control (Con) group, an OGD/R group, and an USW group. The cells in the Con group were cultured without exposure to OGD. The cells in the OGD/R group were treated with OGD/R. The cells in the USW group were treated with USW after OGD/R. Cell morphology was observed under the inverted phase-contrast optical microscope, cell activity was detected by cell counting kit-8 (CCK-8), apoptosis was detected by flow cytometry, and SPCA1 expression was detected by Western blotting. Most of the cells in the Con group showed spindle shape with a clear outline and good adhesion. In the OGD/R group, cells were wrinkled, with blurred outline, poor adhesion, and lots of suspended dead cells appeared; compared with the OGD/R group, the cell morphology and adherence were improved, with clearer outlines and fewer dead cells in the USW group. Compared with the Con group, the OGD/R group showed decreased cell activity, increased apoptotic rate, and down-regulating SPCA1 expression with significant differences (all
ISSN:1672-7347
DOI:10.11817/j.issn.1672-7347.2023.220510