Polyphenols from whole millet grain(Setaria italica) alleviate glucose and lipid homeostasis in diet-induced-obese mice by increasing endogenous GLP-1

• Published in: Journal of the science of food and agriculture 2023-12, Vol.103 (15), p.7785-7797
• Main Authors: An, Yuxuan, Xiaoqin, La, Li, Songtao, Tian, Jinmiao, Fan, Xiaxia, Cui, Kaili, Zhang, Lichao, Li, Zhuoyu
• Format: Article
• Language: English
• Subjects: Body weight gain; Coumaric acid; Diet; Ethanol; Ferulic acid; Glucagon; Glucose; Glucose metabolism; Grain; Homeostasis; Hydroxycinnamic acid; L cells; Lipid metabolism; Lipids; Metabolic disorders; Metabolism; Millet; Phenolic compounds; Phenols; Polyphenols; Purification; Secretion; Setaria italica
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.12901

Foxtail millet (Setaria italica) is a whole millet grain that has been considered for improving the disorder of glucose and lipid metabolism. The purpose of the work is to explore the extraction and enrichment of polyphenols from foxtail millets which can regulate the disorder of glucose and lipid metabolism by increasing endogenous GLP-1(Glucagon-like peptide-1). The optimum ultrasound-assisted extraction (UAE) of foxtail millet polyphenols (FMP) was as follows: 70 °C and 400 W and 70% ethanol concentration, further purification using macroporous resin. In vitro, the FMP eluent of 60% ethanol (FMP-60) has the best effect in promoting GLP-1 secretion from L cells among the different active components of FMP. Millet polyphenols (MP) were obtained from finishing foxtail millet with the bran removed by the same extraction and purification method. Compared with MP-60, FMP-60 mainly included eight active phenolic constituents and contained more ferulic acid, p-coumaric acid, 2-Hydroxycinnamic acid, and Coniferaldehyde. After gavage treatment of DIO mice with FMP-60, FMP-60 promoted endogenous GLP-1 secretion in mice and ameliorated disorders of glucolipid metabolism in DIO mice. FMP-60 could improve glucose homeostasis and ameliorates metabolic disease by promoting the endogenous GLP-1 level and preventing weight gain in diet-induced obese mice. This article is protected by copyright. All rights reserved.