A retrospective analysis of vitamin B6 deficiency and associated changes of gut microbes in Crohn’s disease

• Published in: European journal of clinical nutrition 2023-11, Vol.77 (11), p.1034-1043
• Main Authors: Feng, Zhongsheng, Hua, Jinghan, Guo, Fengjian, Liu, Zhanju, Zhao, Yujie, Wu, Wei
• Format: Article
• Language: English
• Subjects: 692/420/256/2515; 692/699/1503/257/1402; 96/21; Biomarkers; Clinical Nutrition; Crohn's disease; Discriminant analysis; Enzymes; Epidemiology; Feces; Gastroenterology; Genes; Hospitals; Inflammatory bowel disease; Internal Medicine; Laboratories; Mann-Whitney U test; Medicine; Medicine & Public Health; Metabolic Diseases; Metabolism; Microbiota; Microorganisms; Nutrition; Phylogenetics; Public Health; Regression analysis; Software; Statistical analysis; Vitamin B; Vitamin B6; Vitamin deficiency
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/s41430-023-01324-5

Background Patients with inflammatory bowel diseases (IBD) are at risk of micronutrient deficiencies, particularly during flares. Vitamin B6 is required for the proper development of brain, nerves, and many other parts of the body. However, limited studies are available to describe the prevalence, relevance and consequences of vitamin B6 deficiencies in IBD. We aim to estimate the prevalence of vitamin B6 deficiencies in Crohn’s disease (CD) patients, to identify associated risk factors and to explore the alteration of intestinal microbiota related to vitamin B6 status. Methods A total of 360 CD patients and 55 ulcerative colitis (UC) patients from Shanghai Tenth People’s Hospital of Tongji University were included. Serum vitamin B6 concentrations were collected from the computerized laboratory data. The logistic regression was used for statistical analysis. Fecal-associated microbiota was also analyzed using 16S rRNA sequencing in another 20 CD patients (10 of vitamin B6 normal, 10 of vitamin B6 deficiency). Results The prevalence of vitamin B6 abnormality was significantly higher in CD than in UC patients. Logistic regression analysis showed that small bowel lesion, ileocolonic lesion (L3), extraintestinal manifestations, ileal resection, and usage of immunosuppressor were independently associated with abnormal vitamin B6 in CD. Interestingly, the microbial structure presented significant differences between two CD groups. PICRUSt2 prediction revealed that some enzymes and metabolic pathways between the two groups were significantly different. Conclusions Collectively, our analysis showed that vitamin B6 reduction occurred frequently in patients with CD and affected the intestinal flora of patients.