Characterization, in vitro digestibility, antioxidant activity and intestinal peristalsis in zebrafish of Dioscorea opposita polysaccharides

• Published in: International journal of biological macromolecules 2023-10, Vol.250, p.126155-126155, Article 126155
• Main Authors: Wang, Ruijiao, Liu, Wei, Liu, Lu, Ma, Fanyi, Li, Qian, Zhao, Peng, Ma, Wenjing, Cen, Juan, Liu, Xiuhua
• Format: Article
• Language: English
• Subjects: Antioxidant activities; Chinese yam; Simulated gastrointestinal digestion in vitro
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.126155

The soluble crude polysaccharides from Dioscorea opposita (DOP1 and DOP2) were prepared and characterized. DOP1 and DOP2 obtained carbohydrate (65.71% and 70.18%, respectively), uronic acid (63.71% and 24.84%, respectively) and protein (8.09% and 9.51%, respectively) with molecular weight of 49.24 kDa and 21.62 kDa, respectively. DOP samples were mainly composed of mannose, glucose, galacturonic acid, galactose, and glucuronic acid. The digestibility in vitro, antioxidant activity and intestinal peristalsis effect were then investigated. DOP1 and DOP2 were degraded with decreased molecular weights (39.58 kDa and 18.56 kDa respectively), increased reducing sugar contents (from 16.95% to 19.27%; 12.45% to 15.50% respectively) and free monosaccharides (from 0.89% to 1.42%; 0.90% to 1.14% respectively) after gastric digestion. Both DOP1 and DOP2 were resistant to intestinal digestion, suggesting that DOP samples can be considered as a dietary fiber. Additionally, DOP1 and DOP2 exhibited antioxidant activities positively correlated with the concentration and remained the activities after gastrointestinal digestion in vitro. Furthermore, DOP reduced the fluorescence intensity significantly, indicating DOP can promote the intestinal peristalsis of zebrafish larvae (5 pdf) at 500 μg/mL. Therefore, DOP1 and DOP2 have a better functionality as dietary fibers, including antioxidant activity and intestinal peristalsis promotion, which can be developed as functional foods. •Soluble crude polysaccharides from D. opposita (DOP1 and DOP2) were characterized.•DOP samples were degraded in gastric digestion but resistant to intestinal digestion.•DOP1 and DOP2 were considered to be dietary fiber.•DOP1 and DOP2 exhibited antioxidant activities and remained active after digestion.•DOP at 500 μg/mL can promote the intestinal peristalsis in zebrafish larvae.