Lipid Signatures and Inter-Cellular Heterogeneity of Naı̈ve and Lipopolysaccharide-Stimulated Human Microglia-like Cells

Microglia are non-neuronal cells, which reside in the central nervous system and are known to play an important role in health and disease. We investigated the lipidomic phenotypes of human naı̈ve and stimulated microglia-like cells by atmospheric-pressure scanning microprobe matrix-assisted laser d...

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Published in:Analytical chemistry (Washington) 2023-08, Vol.95 (31), p.11672-11679
Main Authors: Müller, Max A., Zweig, Norman, Spengler, Bernhard, Weinert, Maria, Heiles, Sven
Format: Article
Language:English
Subjects:
Analytical chemistry
Cell Differentiation
Cell lines
Central nervous system
Chemistry
Heterogeneity
Humans
Inflammation
Ionization
Lipids
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Mass spectrometry
Mass spectroscopy
Metabolism
Microglia
Phenotypes
Phosphatidylinositols
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Stimulation
Triglycerides
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container_issue 31
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container_title Analytical chemistry (Washington)
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creator Müller, Max A.
Zweig, Norman
Spengler, Bernhard
Weinert, Maria
Heiles, Sven
description Microglia are non-neuronal cells, which reside in the central nervous system and are known to play an important role in health and disease. We investigated the lipidomic phenotypes of human naı̈ve and stimulated microglia-like cells by atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI). With lateral resolutions between 5 and 1.5 μm pixel size, we were able to chart lipid compositions of individual cells, enabling differentiation of cell lines and stimulation conditions. This allowed us to reveal local lipid heterogeneities in naı̈ve and lipopolysaccharide (LPS)-stimulated cells. We were able to identify individual cells with elevated triglyceride (TG) levels and could show that the number of these TG-enriched cells increased with LPS stimulation as a hallmark for a proinflammatory phenotype. Additionally, the observed local abundance alterations of specific phosphatidylinositols (PIs) indicate a cell specific regulation of the PI metabolism.
doi_str_mv 10.1021/acs.analchem.3c01533
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Analytical chemistry
Cell Differentiation
Cell lines
Central nervous system
Chemistry
Heterogeneity
Humans
Inflammation
Ionization
Lipids
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Mass spectrometry
Mass spectroscopy
Metabolism
Microglia
Phenotypes
Phosphatidylinositols
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Stimulation
Triglycerides
title Lipid Signatures and Inter-Cellular Heterogeneity of Naı̈ve and Lipopolysaccharide-Stimulated Human Microglia-like Cells
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