Herbarium specimens reveal century-long trait shifts in poison ivy due to anthropogenic CO2 emissions

• Published in: American journal of botany 2023-09, Vol.110 (9), p.e16225-e16225
• Main Authors: Ng, Molly, Mccormick, Alyssa, Utz, Ryan M, Heberling, J Mason
• Format: Article
• Language: English
• Subjects: Anthropogenic factors; Carbon dioxide; Carbon dioxide emissions; Carbon isotopes; Density; Ecological effects; Leaf area; Leaves; Lianas; Physiological responses; Plant species; Poisons; Species; Stomata; Toxicity; Toxicodendron radicans; Toxicodendron vernix; Water use
• ISSN: 0002-9122; 1537-2197
• DOI: 10.1002/ajb2.16225

Premise Previous experimental studies have shown that poison ivy (Toxicodendron radicans; Anacardicaceae) responds to elevated CO2 with increased leaf production, water-use efficiency, and toxicity (allergenic urushiol). However, long-term field data suggest no increase in poison ivy abundance over time. Using herbarium specimens, we examined whether poison ivy and other species shifted leaf traits under natural conditions with increasing atmospheric CO2 (pCO2) over the past century. Methods We measured stomatal density, leaf area, leaf N, leaf C:N, leaf carbon isotope discrimination (Δleaf), and intrinsic water-use efficiency (iWUE) from 327 specimens collected from 1838 to 2020 across Pennsylvania. We compared poison ivy's responses to two evolutionarily related tree species, Toxicodendron vernix and Rhus typhina (Anacardiacae) and one ecological analog, Parthenocissus quinquefolia (Vitaceae), a common co-occurring liana. Results Stomatal density significantly decreased (P < 0.05) in poison ivy and the ecologically similar liana P. quinquefolia over the past century, but did not change in the related trees T. vernix and R. typhina. None of these species showed significant trends in changes in leaf N or C:N. Surprisingly, in poison ivy, but not the other species, Δleaf increased with increased pCO2, corresponding to significant declines in iWUE over time. Conclusions In contrast to the results of short-term experimental studies, iWUE decreased in poison ivy over the last century. Trait responses to pCO2 varied by species. Herbarium specimens suggest that realized long-term plant physiological responses to increased CO2 may not be reflected in short-term experimental growth studies, highlighting the value of collections.