Methylated tumor suppressor gene SCARA5 inhibits the proliferation, migration and invasion of nasopharyngeal carcinoma

may play an important role in nasopharyngeal carcinoma. PCR and immunohistochemistry were used to detect the expression and promoter methylation of . Cell proliferation assays, spheroid culture, flow cytometry analysis, Transwell assays and xenotransplantation tests were utilized to determine the fu...

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Bibliographic Details
Published in:Epigenomics 2023-06, Vol.15 (11), p.635-650
Main Authors: Jiang, Xianyao, Jiang, Yu, An, Deqiang, Jiang, Xiaocong, Zhou, Shitong, Liu, Yijun, Tian, Rui, Li, Zhuoqing, Zhao, Xunping, Xiang, Tingxiu, Ji, Ping, Yang, Yucheng
Format: Article
Language:English
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Summary:may play an important role in nasopharyngeal carcinoma. PCR and immunohistochemistry were used to detect the expression and promoter methylation of . Cell proliferation assays, spheroid culture, flow cytometry analysis, Transwell assays and xenotransplantation tests were utilized to determine the functional effects of . RNA-sequencing, western blotting, immunofluorescence and dual-luciferase reporter assays were used to assess -mediated outcomes. was downregulated by promoter methylation. Overexpression of inhibited cell migration, invasion and proliferation. enhanced nasopharyngeal carcinoma cell sensitivity to chemotherapy with cisplatin and 5-fluorouracil. drives tumor apoptosis by downregulating . may be a useful clinical marker in nasopharyngeal carcinoma.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2023-0154