DNA damage as an early biomarker of effect in human health

In the last few decades the need for new approaches to assess DNA damage has been increasing due to the implications that different insults on genetic material may have on human health. In this context, the identification of how chemical agents with different mechanisms of action (i.e., antineoplast...

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Bibliographic Details
Published in:Toxicology and industrial health 2005-09, Vol.21 (5-6), p.155-166
Main Authors: Mussali-Galante, P, Avila-Costa, MR, Piñón-Zarate, G, Martínez-Levy, G, Rodríguez-Lara, V, Rojas-Lemus, M, Avila-Casado, MC, Fortoul, TI
Format: Article
Language:English
Subjects:
Antineoplastic Agents - adverse effects
Antineoplastic drugs
Biological
Biomarkers
Comet Assay - methods
Deoxyribonucleic acid
DNA Damage
Drugs
Electrophoresis - methods
Female
Genetics
Health
Human
Humans
Male
Nasal Mucosa - drug effects
Pollutants
Toxicology
Xenobiotics - adverse effects
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Summary:In the last few decades the need for new approaches to assess DNA damage has been increasing due to the implications that different insults on genetic material may have on human health. In this context, the identification of how chemical agents with different mechanisms of action (i.e., antineoplastic drugs) damage DNA provides a good model to investigate some cellular and molecular mechanisms underlying the basis of genetic toxicology. The nasal epithelium is the first barrier with which environmental pollutants interact, and for this reason this epithelium can be useful as a sentinel in order to assess the interactions between the environment and the living organisms. Taking these phenomena into account and using a simple, sensitive and rapid method such as the single cell gel electrophoresis, we could obtain information and an initial approach on the DNA status. This assay in combination with other techniques that provide more information about other molecular parameters could give us a better view of the biological status of the living cell.
ISSN:0748-2337
1477-0393
DOI:10.1191/0748233705th224oa