Site-specific response of sediment microbial community to supplementation of polyhydroxyalkanoates as biostimulants for PCB reductive dechlorination

• Published in: The Science of the total environment 2023-11, Vol.898, p.165485-165485, Article 165485
• Main Authors: Botti, Alberto, Musmeci, Eliana, Negroni, Andrea, Capuozzo, Rosaria, Fava, Fabio, Biagi, Elena, Zanaroli, Giulio
• Format: Article
• Language: English
• Subjects: biodegradability; Biodegradation; biodiversity; Bioplastics; dechlorination; environment; hydrogen; Italy; Marine sediments; microbial communities; Microbiome; Polyhydroxyalkanoates
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.165485

The use of biodegradable plastics is constantly raising, increasing the likeliness for these polymers to end up in the environment. Environmental applications foreseeing the intentional release of biodegradable plastics have been also recently proposed, e.g., for polyhydroxyalkanoates (PHAs) acting as slow hydrogen releasing compounds to stimulate microbial reductive dehalogenation processes. However, the effects of their release into the environment on the ecosystems still need to be thoroughly explored. In this work, the use of PHAs to enhance the microbial reductive dechlorination of polychlorobiphenyls (PCBs) and their impact on the metabolic and compositional features of the resident microbial community have been investigated in laboratory microcosms of a polluted marine sediment from Mar Piccolo (Taranto, Italy), and compared with recent findings on a different contaminated marine sediment from Pialassa della Baiona (Ravenna, Italy). A decreased biostimulation efficiency of PHAs on PCBs reductive dechlorination was observed in the sediment from Mar Piccolo, with respect to the sediment from Pialassa della Baiona, suggesting that the sediments' physical-chemical characteristics and/or the biodiversity and composition of its microbial community might play a key role in determining the outcome of this biostimulation strategy. Regardless of the sediment origin, PHAs were found to have a specific and pervasive effect on the sediment microbial community, reducing its biodiversity, defining a newly arranged microbial core of primary degraders and consequently affecting, in a site-specific way, the abundance of subdominant bacteria, possibly cross-feeders. Such potential to dramatically change the structure of autochthonous microbial communities should be carefully considered, since it might have secondary effects, e.g., on the natural biogeochemical cycles. [Display omitted] •Efficacy of PHA biostimulation of PCB dechlorination depends on sediment's features.•Biodegradable polymers entering marine sediments impact the resident microbiome.•PHA as carbon source selects for abundant microbial primary degraders.•Site-specific signatures persist in subdominant sediment microbiome members.