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Heterologous expression of cry1Ia12 insecticidal gene in cotton encodes resistance against pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae); an alternate insecticidal gene for insect pest management
Background Cotton is continuously exposed to sucking and chewing insect pest pressure since emergence to harvesting. Pink bollworm ( Pectinophora gossypiella ) has become major chewing insect pest to reduce the cotton yield and results in bad lint quality even in transgenic crops. The efficiency of...
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| Published in: | Molecular biology reports 2022-11, Vol.49 (11), p.10557-10564 |
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| creator | Tariq, Muhammad Tabassum, Bushra Bakhsh, Allah Farooq, Abdul Munim Qamar, Zahida Akram, Faheem Naz, Farah Rao, Abdul Qayyum Malik, Kausar Nasir, Idrees Ahmad |
| description | Background
Cotton is continuously exposed to sucking and chewing insect pest pressure since emergence to harvesting. Pink bollworm (
Pectinophora gossypiella
) has become major chewing insect pest to reduce the cotton yield and results in bad lint quality even in transgenic crops. The efficiency of insecticidal genes has been compromised due to extensive utilization of transgenic crops.
Methods and Results
The present study was conducted to evaluate the efficacy of an alternate
cry1Ia12
insecticidal gene against pink bollworm (PBW) in cotton.
Agrobacterium tumefaciens
strain LBA4404 harboring pCAMBIA2300 expression vector containing
cry1Ia12
gene under the control of 35S CaMV was used to transform a local cotton cultivar GS-01. The various molecular analyses revealed the transgene integration and expression in primary transformants. Among five selected transgenic plants, tcL-08 showed maximum (16.06-fold) mRNA expression of
cry1Ia12
gene whereas tcL-03 showed minimum (2.33-fold) expression. Feeding bioassays of 2nd and 3rd instar pink bollworm (PBW) larvae on immature cotton bolls, flowers and cotton squares revealed up to 33.33% mortality on tcL-08 while lowest mortality (13.33%) was observed in tcL-03 and tcL-15. Furthermore, the average weight and size of survived larvae fed on transgenic plants was significantly lesser than the average weight of larvae survived on non-transgenic plants.
Conclusions
The present study suggests the
cry1Ia12
gene as an alternate insecticidal gene for the resistance management of cotton bollworms, especially PBW. |
| doi_str_mv | 10.1007/s11033-022-07824-0 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2849900814</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2718962808</sourcerecordid><originalsourceid>FETCH-LOGICAL-c336t-992b92c7d77745771f2e70826f66f68f79b6bb3f03faa8c27bf5de2488abe6083</originalsourceid><addsrcrecordid>eNqFkt-K1DAUxosoOK6-gFcBb1awepK0TapXy-L-gQG90OuSpifdrGlSkww6b-rjbGZnQRBRCASS3_edk5Ovql5SeEsBxLtEKXBeA2M1CMmaGh5VG9oKXje9kI-rDXCgdSNb-rR6ltItADRUtJvq1xVmjMGFOewSwZ9rxJRs8CQYouOeXivKiPUJdbbaTsqRGT2WE6JDzoVDr8OEiRSdTVl5jUTNqigyWa3_Rsbg3I8Qlzfk88HDh_UmREXmkNJ-teicIqdbXO0U1tKIek8u0aG-saUWvv5AlCfKlQuvMv6lDxPiwylZsZRclFczLujz8-qJUS7hi4f9pPp68fHL-VW9_XR5fX62rTXnXa77no0902ISQjStENQwFCBZZ7qypBH92I0jN8CNUlIzMZp2QtZIqUbsQPKT6vTou8bwfVd6GBab9OFdHstIByabvgeQtPk_KqjsOybvXV_9gd6GXRmCO1DlK0UPrCsUO1I6lnFGNMMa7aLifqAwHHIxHHMxlFwM97kYoIj4UZQK7GeMv63_oboDT5e_mQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2730179026</pqid></control><display><type>article</type><title>Heterologous expression of cry1Ia12 insecticidal gene in cotton encodes resistance against pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae); an alternate insecticidal gene for insect pest management</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Tariq, Muhammad ; Tabassum, Bushra ; Bakhsh, Allah ; Farooq, Abdul Munim ; Qamar, Zahida ; Akram, Faheem ; Naz, Farah ; Rao, Abdul Qayyum ; Malik, Kausar ; Nasir, Idrees Ahmad</creator><creatorcontrib>Tariq, Muhammad ; Tabassum, Bushra ; Bakhsh, Allah ; Farooq, Abdul Munim ; Qamar, Zahida ; Akram, Faheem ; Naz, Farah ; Rao, Abdul Qayyum ; Malik, Kausar ; Nasir, Idrees Ahmad</creatorcontrib><description>Background
Cotton is continuously exposed to sucking and chewing insect pest pressure since emergence to harvesting. Pink bollworm (
Pectinophora gossypiella
) has become major chewing insect pest to reduce the cotton yield and results in bad lint quality even in transgenic crops. The efficiency of insecticidal genes has been compromised due to extensive utilization of transgenic crops.
Methods and Results
The present study was conducted to evaluate the efficacy of an alternate
cry1Ia12
insecticidal gene against pink bollworm (PBW) in cotton.
Agrobacterium tumefaciens
strain LBA4404 harboring pCAMBIA2300 expression vector containing
cry1Ia12
gene under the control of 35S CaMV was used to transform a local cotton cultivar GS-01. The various molecular analyses revealed the transgene integration and expression in primary transformants. Among five selected transgenic plants, tcL-08 showed maximum (16.06-fold) mRNA expression of
cry1Ia12
gene whereas tcL-03 showed minimum (2.33-fold) expression. Feeding bioassays of 2nd and 3rd instar pink bollworm (PBW) larvae on immature cotton bolls, flowers and cotton squares revealed up to 33.33% mortality on tcL-08 while lowest mortality (13.33%) was observed in tcL-03 and tcL-15. Furthermore, the average weight and size of survived larvae fed on transgenic plants was significantly lesser than the average weight of larvae survived on non-transgenic plants.
Conclusions
The present study suggests the
cry1Ia12
gene as an alternate insecticidal gene for the resistance management of cotton bollworms, especially PBW.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07824-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agrobacterium radiobacter ; Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Chewing ; Cotton ; Crops ; Cultivars ; Flowers ; Gene expression ; genetic vectors ; genetically modified organisms ; Harvesting ; Helicoverpa armigera ; heterologous gene expression ; Histology ; insect control ; insect pests ; instars ; Larvae ; Life Sciences ; lint cotton ; Morphology ; Mortality ; Original Article ; Pectinophora gossypiella ; Pest control ; Pest resistance ; resistance management ; transgenes ; Transgenic plants</subject><ispartof>Molecular biology reports, 2022-11, Vol.49 (11), p.10557-10564</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c336t-992b92c7d77745771f2e70826f66f68f79b6bb3f03faa8c27bf5de2488abe6083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Tariq, Muhammad</creatorcontrib><creatorcontrib>Tabassum, Bushra</creatorcontrib><creatorcontrib>Bakhsh, Allah</creatorcontrib><creatorcontrib>Farooq, Abdul Munim</creatorcontrib><creatorcontrib>Qamar, Zahida</creatorcontrib><creatorcontrib>Akram, Faheem</creatorcontrib><creatorcontrib>Naz, Farah</creatorcontrib><creatorcontrib>Rao, Abdul Qayyum</creatorcontrib><creatorcontrib>Malik, Kausar</creatorcontrib><creatorcontrib>Nasir, Idrees Ahmad</creatorcontrib><title>Heterologous expression of cry1Ia12 insecticidal gene in cotton encodes resistance against pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae); an alternate insecticidal gene for insect pest management</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Background
Cotton is continuously exposed to sucking and chewing insect pest pressure since emergence to harvesting. Pink bollworm (
Pectinophora gossypiella
) has become major chewing insect pest to reduce the cotton yield and results in bad lint quality even in transgenic crops. The efficiency of insecticidal genes has been compromised due to extensive utilization of transgenic crops.
Methods and Results
The present study was conducted to evaluate the efficacy of an alternate
cry1Ia12
insecticidal gene against pink bollworm (PBW) in cotton.
Agrobacterium tumefaciens
strain LBA4404 harboring pCAMBIA2300 expression vector containing
cry1Ia12
gene under the control of 35S CaMV was used to transform a local cotton cultivar GS-01. The various molecular analyses revealed the transgene integration and expression in primary transformants. Among five selected transgenic plants, tcL-08 showed maximum (16.06-fold) mRNA expression of
cry1Ia12
gene whereas tcL-03 showed minimum (2.33-fold) expression. Feeding bioassays of 2nd and 3rd instar pink bollworm (PBW) larvae on immature cotton bolls, flowers and cotton squares revealed up to 33.33% mortality on tcL-08 while lowest mortality (13.33%) was observed in tcL-03 and tcL-15. Furthermore, the average weight and size of survived larvae fed on transgenic plants was significantly lesser than the average weight of larvae survived on non-transgenic plants.
Conclusions
The present study suggests the
cry1Ia12
gene as an alternate insecticidal gene for the resistance management of cotton bollworms, especially PBW.</description><subject>Agrobacterium radiobacter</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Chewing</subject><subject>Cotton</subject><subject>Crops</subject><subject>Cultivars</subject><subject>Flowers</subject><subject>Gene expression</subject><subject>genetic vectors</subject><subject>genetically modified organisms</subject><subject>Harvesting</subject><subject>Helicoverpa armigera</subject><subject>heterologous gene expression</subject><subject>Histology</subject><subject>insect control</subject><subject>insect pests</subject><subject>instars</subject><subject>Larvae</subject><subject>Life Sciences</subject><subject>lint cotton</subject><subject>Morphology</subject><subject>Mortality</subject><subject>Original Article</subject><subject>Pectinophora gossypiella</subject><subject>Pest control</subject><subject>Pest resistance</subject><subject>resistance management</subject><subject>transgenes</subject><subject>Transgenic plants</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkt-K1DAUxosoOK6-gFcBb1awepK0TapXy-L-gQG90OuSpifdrGlSkww6b-rjbGZnQRBRCASS3_edk5Ovql5SeEsBxLtEKXBeA2M1CMmaGh5VG9oKXje9kI-rDXCgdSNb-rR6ltItADRUtJvq1xVmjMGFOewSwZ9rxJRs8CQYouOeXivKiPUJdbbaTsqRGT2WE6JDzoVDr8OEiRSdTVl5jUTNqigyWa3_Rsbg3I8Qlzfk88HDh_UmREXmkNJ-teicIqdbXO0U1tKIek8u0aG-saUWvv5AlCfKlQuvMv6lDxPiwylZsZRclFczLujz8-qJUS7hi4f9pPp68fHL-VW9_XR5fX62rTXnXa77no0902ISQjStENQwFCBZZ7qypBH92I0jN8CNUlIzMZp2QtZIqUbsQPKT6vTou8bwfVd6GBab9OFdHstIByabvgeQtPk_KqjsOybvXV_9gd6GXRmCO1DlK0UPrCsUO1I6lnFGNMMa7aLifqAwHHIxHHMxlFwM97kYoIj4UZQK7GeMv63_oboDT5e_mQ</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Tariq, Muhammad</creator><creator>Tabassum, Bushra</creator><creator>Bakhsh, Allah</creator><creator>Farooq, Abdul Munim</creator><creator>Qamar, Zahida</creator><creator>Akram, Faheem</creator><creator>Naz, Farah</creator><creator>Rao, Abdul Qayyum</creator><creator>Malik, Kausar</creator><creator>Nasir, Idrees Ahmad</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20221101</creationdate><title>Heterologous expression of cry1Ia12 insecticidal gene in cotton encodes resistance against pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae); an alternate insecticidal gene for insect pest management</title><author>Tariq, Muhammad ; Tabassum, Bushra ; Bakhsh, Allah ; Farooq, Abdul Munim ; Qamar, Zahida ; Akram, Faheem ; Naz, Farah ; Rao, Abdul Qayyum ; Malik, Kausar ; Nasir, Idrees Ahmad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-992b92c7d77745771f2e70826f66f68f79b6bb3f03faa8c27bf5de2488abe6083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agrobacterium radiobacter</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Chewing</topic><topic>Cotton</topic><topic>Crops</topic><topic>Cultivars</topic><topic>Flowers</topic><topic>Gene expression</topic><topic>genetic vectors</topic><topic>genetically modified organisms</topic><topic>Harvesting</topic><topic>Helicoverpa armigera</topic><topic>heterologous gene expression</topic><topic>Histology</topic><topic>insect control</topic><topic>insect pests</topic><topic>instars</topic><topic>Larvae</topic><topic>Life Sciences</topic><topic>lint cotton</topic><topic>Morphology</topic><topic>Mortality</topic><topic>Original Article</topic><topic>Pectinophora gossypiella</topic><topic>Pest control</topic><topic>Pest resistance</topic><topic>resistance management</topic><topic>transgenes</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tariq, Muhammad</creatorcontrib><creatorcontrib>Tabassum, Bushra</creatorcontrib><creatorcontrib>Bakhsh, Allah</creatorcontrib><creatorcontrib>Farooq, Abdul Munim</creatorcontrib><creatorcontrib>Qamar, Zahida</creatorcontrib><creatorcontrib>Akram, Faheem</creatorcontrib><creatorcontrib>Naz, Farah</creatorcontrib><creatorcontrib>Rao, Abdul Qayyum</creatorcontrib><creatorcontrib>Malik, Kausar</creatorcontrib><creatorcontrib>Nasir, Idrees Ahmad</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 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Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tariq, Muhammad</au><au>Tabassum, Bushra</au><au>Bakhsh, Allah</au><au>Farooq, Abdul Munim</au><au>Qamar, Zahida</au><au>Akram, Faheem</au><au>Naz, Farah</au><au>Rao, Abdul Qayyum</au><au>Malik, Kausar</au><au>Nasir, Idrees Ahmad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous expression of cry1Ia12 insecticidal gene in cotton encodes resistance against pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae); an alternate insecticidal gene for insect pest management</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><date>2022-11-01</date><risdate>2022</risdate><volume>49</volume><issue>11</issue><spage>10557</spage><epage>10564</epage><pages>10557-10564</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Cotton is continuously exposed to sucking and chewing insect pest pressure since emergence to harvesting. Pink bollworm (
Pectinophora gossypiella
) has become major chewing insect pest to reduce the cotton yield and results in bad lint quality even in transgenic crops. The efficiency of insecticidal genes has been compromised due to extensive utilization of transgenic crops.
Methods and Results
The present study was conducted to evaluate the efficacy of an alternate
cry1Ia12
insecticidal gene against pink bollworm (PBW) in cotton.
Agrobacterium tumefaciens
strain LBA4404 harboring pCAMBIA2300 expression vector containing
cry1Ia12
gene under the control of 35S CaMV was used to transform a local cotton cultivar GS-01. The various molecular analyses revealed the transgene integration and expression in primary transformants. Among five selected transgenic plants, tcL-08 showed maximum (16.06-fold) mRNA expression of
cry1Ia12
gene whereas tcL-03 showed minimum (2.33-fold) expression. Feeding bioassays of 2nd and 3rd instar pink bollworm (PBW) larvae on immature cotton bolls, flowers and cotton squares revealed up to 33.33% mortality on tcL-08 while lowest mortality (13.33%) was observed in tcL-03 and tcL-15. Furthermore, the average weight and size of survived larvae fed on transgenic plants was significantly lesser than the average weight of larvae survived on non-transgenic plants.
Conclusions
The present study suggests the
cry1Ia12
gene as an alternate insecticidal gene for the resistance management of cotton bollworms, especially PBW.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11033-022-07824-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular biology reports, 2022-11, Vol.49 (11), p.10557-10564 |
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| source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
| subjects | Agrobacterium radiobacter Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Chewing Cotton Crops Cultivars Flowers Gene expression genetic vectors genetically modified organisms Harvesting Helicoverpa armigera heterologous gene expression Histology insect control insect pests instars Larvae Life Sciences lint cotton Morphology Mortality Original Article Pectinophora gossypiella Pest control Pest resistance resistance management transgenes Transgenic plants |
| title | Heterologous expression of cry1Ia12 insecticidal gene in cotton encodes resistance against pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae); an alternate insecticidal gene for insect pest management |
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