Genotyping of Macaque Population on the Brain-Derived Neurotrophic Factor Gene Polymorphism by Mismatch Amplification Mutation Assay (MAMA)-PCR

• Published in: Bulletin of experimental biology and medicine 2023-07, Vol.175 (3), p.388-392
• Main Authors: Pavlova, L. E., Timina, M. F., Agumava, A. A., Panchenko, A. V.
• Format: Article
• Language: English
• Subjects: Alleles; Animals; Biomedical and Life Sciences; Biomedicine; Brain research; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - genetics; Cell Biology; Gene polymorphism; Genes; Genetic testing; Genotype; Genotyping; Humans; Internal Medicine; Laboratory Medicine; Macaca fascicularis - genetics; Macaca mulatta - genetics; Male; Mutation; Mutation - genetics; Pathology; Polymerase Chain Reaction; Polymorphism; Polymorphism, Genetic - genetics; Primatology; Research centers; Single nucleotide polymorphisms; Software
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-023-05873-4

Human rs6265 ( 196G>A ) polymorphism in the BDNF gene is associated with many clinically significant phenotypic manifestations. Rhesus monkey ( Macaca mulatta ) has a functionally significant rs309950446 ( 136G>A ) polymorphism. To determine this polymorphism in macaques, we used mismatch amplification mutation assay (MAMA)-PCR method with non-complementary nucleotide to the template chain at the 3rd position from the 3’-end of the allele-specific primers (mismatch primers), which allowed the best discrimination of the alleles. Genotyping of male rhesus monkeys ( n =178) and cynomolgus monkeys ( Macaca fascicularis ) ( n =90) was carried out. The A/A , G/G , and G/A genotypes were found in 16, 34, and 50% rhesus macaques, respectively. In the cynomolgus macaques, the mutant polymorphic allele was not detected. The study results allow considering rhesus macaques as a potential biological model for assessment of the gen—environment interaction of the BDNF gene polymorphism.