Ectopic expression of Camellia oleifera Abel. gibberellin 20-oxidase gene increased plant height and promoted secondary cell walls deposition in Arabidopsis

• Published in: Planta 2023-09, Vol.258 (3), p.65-65, Article 65
• Main Authors: Wang, Ying, Li, Jian’an, Guo, Purui, Liu, Qian, Ren, Shuangshuang, Juan, Lemei, He, Jiacheng, Tan, Xiaofeng, Yan, Jindong
• Format: Article
• Language: English
• Subjects: Accumulation; Agriculture; Amino acids; Anthocyanins; Arabidopsis; Biomedical and Life Sciences; Biosynthesis; Camellia oleifera; Cell walls; Chlorophyll; Ecology; Ectopic expression; Enlargement; Flowering; Forestry; Genetic analysis; Genotype & phenotype; Gibberellins; Leaves; Life Sciences; Molecular modelling; Original Article; Oxidase; Phenotypes; Plant Sciences; Plants (botany); Transgenic plants; Wall deposition
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-023-04222-z

Main conclusion Ectopic expression of Camellia oleifera Abel. gibberellin 20-oxidase 1 caused a taller phenotype, promoted secondary cell wall deposition, leaf enlargement, and early flowering, and reduced chlorophyll and anthocyanin accumulation and seed enlargement phenotype in Arabidopsis . Plant height and secondary cell wall (SCW) deposition are important plant traits. Gibberellins (GAs) play important roles in regulating plant height and SCWs deposition. Gibberellin 20-oxidase (GA20ox) is an important enzyme involved in GA biosynthesis. In the present study, we identified a GA synthesis gene in Camellia oleifera. The total length of the CoGA20ox1 gene sequence was 1146 bp, encoding 381 amino acids. Transgenic plants with CoGA20ox1 had a taller phenotype; a seed enlargement phenotype; promoted SCWs deposition, leaf enlargement, and early flowering; and reduced chlorophyll and anthocyanin accumulation. Genetic analysis showed that the mutant ga20ox1-3 Arabidopsis partially rescued the phenotype of CoGA20ox1 overexpression plants. The results showed that CoGA20ox1 participates in the growth and development of C. oleifera. The morphological changes in CoGA20ox1 overexpressed plants provide a theoretical basis for further exploration of GA biosynthesis and analysis of the molecular mechanism in C. oleifera.