Comprehensive analysis of transcriptional data on seed germination of two maize inbred lines under low-temperature conditions

• Published in: Plant physiology and biochemistry 2023-08, Vol.201, p.107874-107874, Article 107874
• Main Authors: Zhang, Yinchao, Liu, Peng, Zou, Chaoying, Chen, Zhong, Yuan, Guangsheng, Gao, Shibin, Pan, Guangtang, Shen, Yaou, Ma, Langlang
• Format: Article
• Language: English
• Subjects: Hub genes; Low-temperature; Maize; RNA-Seq; Seed germination
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2023.107874

Seed germination directly affect maize yield and grain quality. Low-temperature reduces maize yield by affecting seed germination and seedling growth. However, the molecular mechanism of maize seed germination under low-temperature remains unclear. In this study, the transcriptome data of two maize inbred lines SCL127 (chilling-sensitive) and SCL326 (chilling-tolerant) were analyzed at five time points (0 H, 4 H, 12 H, 24 H, and 48 H) under low-temperature conditions. Through the comparison of SCL127-0 H-vs-SCL326-0 H (Group I), SCL127-4 H-vs-SCL326-4 H (Group Ⅱ), SCL127-12 H-vs-SCL326-12 H (Group Ⅲ), SCL127-24 H-vs-SCL326-24 H (Group Ⅳ), and SCL127-48 H-vs SCL326-48 H (Group Ⅴ), a total of 8,526 differentially expressed genes (DEGs) were obtained. Weighted correlation network analysis revealed that Zm00001d010445 was the hub gene involved in seed germination under low-temperature conditions. Zm00001d010445-based association analysis showed that Hap Ⅱ (G) was the excellent haplotype for seed germination under low-temperature conditions. These findings provide a new perspective for the study of the genetic architecture of maize tolerance to low-temperature and contribute to the cultivation of maize varieties with low-temperature tolerance. •RNA-seq was performed on two maize lines under low-temperature.•Zm00001d010445 was identified to affect maize germination.