Polyamidoamine with a hyper-branched structure grafted on modified magnetic graphene oxide for the trace separation of diclofenac and acetaminophen followed by high-performance liquid chromatography determination

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2023-07, Vol.1227, p.123845-123845, Article 123845
• Main Authors: Dehghani Soltani, Maryam, Mohammad Haji Shabani, Ali, Dadfarnia, Shayessteh, Tamaddon, Fatemah
• Format: Article
• Language: English
• Subjects: Acetaminophen; Diclofenac; Dispersive magnetic solid-phase microextraction; High-performance liquid chromatography; Polyamidoamine dendrimers
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2023.123845

•- MGO was modified with 3-aminopropyltriethoxysilane to improve its adhesion to PAMAM.•- Synthesis of different generations of PAMAM dendrimers on MGO modified with APTES.•- Application of PAMAM dendrimers grafted on modified MGO for the extraction of NSAIDs. Different generations of polyamidoamine dendrimers were synthesized on a focal core of magnetic graphene oxide modified with 3-aminopropyltriethoxysilane. After the characterization of synthesized dendrimers, its second generation was employed as a suitable sorbent for simultaneous separation/preconcentration of diclofenac and acetaminophen by a dispersive magnetic solid phase microextraction. The extracted analytes were then quantified by high-performance liquid chromatography with ultraviolet detection. Under optimized conditions, the limits of detection were 0.3 µg L-1 for diclofenac and 0.1 µg L-1 for acetaminophen. The intra-day relative standard deviations at 50 μg L-1 levels were 1.8% for diclofenac and 2.1% for acetaminophen, while the inter-day relative standard deviations were 3.6% and 4.5% for diclofenac and acetaminophen, respectively. The calibration graphs were linear in ranges of 1.0–500.0 µg L-1 and 0.5-600.0 µg L-1 for diclofenac and acetaminophen, respectively, with good coefficients of determination (r2 > 0.998). The method was successfully applied to the determination of diclofenac and acetaminophen in water, milk, and biological samples.