OsbHLH92, in the noncanonical brassinosteroid signaling pathway, positively regulates leaf angle and grain weight in rice

Summary Modifications of plant architecture can increase planting density, regulate photosynthesis, and improve crop yields. Many basic helix–loop–helix (bHLH) transcription factors participate in the brassinosteroid (BR) signaling pathway and are critical for plant architecture morphogenesis in ric...

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Published in:The New phytologist 2023-11, Vol.240 (3), p.1066-1081
Main Authors: Teng, Shouzhen, Liu, Qiming, Chen, Guoxin, Chang, Yuan, Cui, Xuean, Wu, Jinxia, Ai, Pengfei, Sun, Xuehui, Zhang, Zhiguo, Lu, Tiegang
Format: Article
Language:English
Subjects:
brassinosteroid
Cell size
Crop production
Crop yield
Genes
Helix-loop-helix proteins (basic)
Homology
Leaf angle
Leaves
Morphogenesis
Parenchyma
parenchyma cell layers
Photosynthesis
Planting
Planting density
Plants (botany)
Productivity
Rice
Signal transduction
Transcription
Transcription factors
typical bHLH transcription factors
yield
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Summary:Summary Modifications of plant architecture can increase planting density, regulate photosynthesis, and improve crop yields. Many basic helix–loop–helix (bHLH) transcription factors participate in the brassinosteroid (BR) signaling pathway and are critical for plant architecture morphogenesis in rice. However, the number of identified bHLH genes suitable for improving production value is still limited. In this study, we cloned Lam1, encoding the typical bHLH transcription factor OsbHLH92. OsbHLH92 knockout (KO) lines exhibit erect leaves. Decreases in the number and size of parenchyma cell layers on the adaxial side of the lamina joint in KO lines were the main reason for the decreased leaf angle. Genetic experiments verify that OsBU1 and its homologs are downstream of OsbHLH92, which is involved in the noncanonical RGA1‐mediated BR signaling pathway. OsbHLH91, an OsbHLH92 homolog, plays both conserved and differentiated roles relative to OsbHLH92. Notably, OsbHLH92‐KO lines show erect leaves without the acquisition of adverse agronomic traits. Moreover, by driving a specific panicle promoter, OsbHLH92 can greatly increase productivity by at least 10%. This study identifies new components of the BR signaling pathway, demonstrates the importance of OsbHLH92 in improving planting density and crop productivity, and broadens our knowledge of typical and atypical bHLH family members in rice.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.19204