Laccase improvement in submerged cultivation: induced production and kinetic modelling

Improvement of laccase production by Trametes versicolor was made by employing different operational strategies. In the cell growth medium, various glucose concentrations were compared for improving laccase production. A clear and significant stimulation of enzyme production under carbon limitation...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2005-06, Vol.80 (6), p.669-676
Main Authors: Tavares, A.P.M, Coelho, M.A.Z, Coutinho, J.A.P, Xavier, A.M.R.B
Format: Article
Language:English
Subjects:
Agricultural and forest climatology and meteorology. Irrigation. Drainage
Agricultural and forest meteorology
Agronomy. Soil science and plant productions
Biological and medical sciences
biosynthesis
carbon limitation
cell suspension culture
copper
Coriolus versicolor
culture media
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Generalities. Techniques. Climatology. Meteorology. Climatic models of plant production
inducers
laccase
modelling
nutrient availability
phenolic compounds
Trametes versicolor
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Summary:Improvement of laccase production by Trametes versicolor was made by employing different operational strategies. In the cell growth medium, various glucose concentrations were compared for improving laccase production. A clear and significant stimulation of enzyme production under carbon limitation was obtained. Copper, 2,5‐xylidine, and a phenolic mixture were also used as laccase inducers. A cooperative effect between the inducers on laccase production was identified. Mixtures of inducers produced higher laccase activities, reaching values of 5500 U dm−3. Further productivity enhancement can be obtained using the inducers along with the carbon limitation strategy. It is shown that low laccase concentrations are obtained by a primary metabolism of T versicolor, and that phenolic compounds and carbon limitation induce a secondary metabolism, providing higher laccase concentrations. A mathematical model for laccase production based on a direct experimental measure of biomass, along with substrate consumption and enzymatic activity over time is proposed for non‐homogeneous fermentations of T versicolor. Copyright © 2005 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.1246