Abietane diterpenoids with anti-inflammatory activities from Callicarpa bodinieri

• Published in: Phytochemistry (Oxford) 2023-10, Vol.214, p.113825-113825, Article 113825
• Main Authors: Sun, Yue, Yang, Hong-Ying, Huang, Pei-Zhi, Zhang, Lan-Jun, Feng, Wei-Jiao, Li, Ya, Gao, Kun
• Format: Article
• Language: English
• Subjects: Abietane-type diterpenoids; Anti-inflammatory activities; Callicarpa bodinieri; Lamiaceae
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2023.113825

Nine undescribed abietane diterpenoids (1–9) and eleven known abietane analogs (10–20) were isolated from Callicarpa bodinieri. Their structures were characterized by interpreting spectroscopic data, X-ray crystallography, and ECD analysis. The anti-inflammatory activities of these compounds were tested by evaluation of their inhibitory effect on NO production by lipopolysaccharide in RAW 264.7 macrophages, and compounds 3 and 8 exhibited potent anti-inflammatory activities with IC50 values of 36.35 ± 1.12 and 37.21 ± 0.92 μM. The western blotting studies demonstrated that compound 3 inhibited the expression of nitric oxide synthase and p65 that involved in the NF-κB pathway. Nine undescribed abietane type diterpenoids were isolated from Callicarpa bodinieri, and compound 3 significantly inhibited the expression of nitric oxide synthase and p65. [Display omitted] •Nine undescribed abietane type diterpenoids and eleven known ones were isolated.•Two compounds showed potent inhibitory effects on NO production.•Compound 3 significantly inhibited the expression of nitric oxide synthase and p65.