Isolation, identification of antioxidant peptides from earthworm proteins and analysis of the structure–activity relationship of the peptides based on quantum chemical calculations

••Gastrointestinal digestive products of earthworm protein has antioxidant activity.••Antioxidant peptides in the digestive products were isolated and identified.•• The peptides, AFWYGLPCKL, GCFRYACGAFY, WPWQMSLY showed good antioxidant activities.•• The structure–activity relationships of the three...

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Bibliographic Details
Published in:Food chemistry 2024-01, Vol.431, p.137137-137137, Article 137137
Main Authors: He, Ping, Zhang, Yi, Zhang, Yizhe, Zhang, Lina, Lin, ZhengLi, Sun, Chongzhen, Wu, Hui, Zhang, Mengmeng
Format: Article
Language:English
Subjects:
Active site
Antioxidant peptide
Earthworm
Protein
Quantum chemistry
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Summary:••Gastrointestinal digestive products of earthworm protein has antioxidant activity.••Antioxidant peptides in the digestive products were isolated and identified.•• The peptides, AFWYGLPCKL, GCFRYACGAFY, WPWQMSLY showed good antioxidant activities.•• The structure–activity relationships of the three peptides were analyzed. Earthworms are emerging sources of edible animal proteins. Earthworm extracts exhibit good in vivo antioxidant activity after oral administration. To better understand the antioxidant activity of earthworms, antioxidant peptides derived from earthworm proteins after gastrointestinal digestion were isolated and identified, and their structure–activity relationships were analysed in this research. Results showed that earthworm protein gastrointestinal digestion products exhibited good antioxidant activity, and 6030 peptide sequences were identified after separation using ion-exchange and gel-chromatography columns. Eleven peptides were screened using computer simulation activity scores, among which AFWYGLPCKL, WPWQMSLY, and GCFRYACGAFY showed the best antioxidant activities. Highest Occupied Molecular Orbital (HOMO) analysis indicated that N29-H10, O122-H38, and the peptide bond binding sites of serine and leucine were active sites of peptides AFWYGLPCKL, GCFRYACGAFY, and WPWQMSLY, respectively. This study provides a new understanding of substance basis of antioxidant activity in earthworms and contributes to application of earthworm proteins as antioxidants in health-foods.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2023.137137