An electrochemical approach for the prediction of Δ9-tetrahydrocannabinolic acid and total cannabinoid content in Cannabis sativa L

• Published in: Analyst (London) 2023-09, Vol.148 (19), p.4688-4697
• Main Authors: Monari, Alessandro, Cantalù, Sara, Zanfrognini, Barbara, Brighenti, Virginia, Verri, Patrizia, Zanardi, Chiara, Pellati, Federica, Pigani, Laura
• Format: Article
• Language: English
• Subjects: Cannabis; Chemical sensors; Electrode materials; Electrodes; High performance liquid chromatography
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d3an01090b

Two electrochemical sensors are proposed here for the first time for the fast screening of cannabinoids in Cannabis sativa L. plant material (inflorescences). The accurate control of cannabinoid content is important for discriminating between recreational, i.e. illegal, and fibre-type C. sativa samples, which differ mainly according to the amount of Δ9-tetrahydrocannabinol (Δ9-THC) and Δ9-tetrahydrocannabinolic acid (Δ9-THCA). Two screen printed electrodes obtained using different electrode materials were tested for the analysis of extracts from recreational and fibre-type C. sativa and their performance was compared with a consolidated method based on high-performance liquid chromatography (HPLC). The voltammetric responses recorded in the different samples reflected the compositional differences of the recreational and fibre-type extracts in accordance with the results of HPLC analyses. Moreover, the quantification of Δ9-THCA and the total cannabinoid content on the basis of the intensity of the peaks of the voltammograms was possible through a simple and fast electrochemical procedure.