Photochemical outcomes triggered by gold shell-isolated nanorods on bioinspired nanoarchitectonics for bacterial membranes

• Published in: Biochimica et biophysica acta. Biomembranes 2023-12, Vol.1865 (8), p.184216-184216, Article 184216
• Main Authors: Almeida Jr, Alexandre M., Moreira, Lucas G., Camacho, Sabrina A., Ferreira, Fabiana G., Conceição, Katia, Tada, Dayane B., Aoki, Pedro H.B.
• Format: Article
• Language: English
• Subjects: Bioinspired bacterial membranes; Gold shell-isolated nanorods (AuSHINRs); Photothermal therapy
• ISSN: 0005-2736; 1879-2642
• DOI: 10.1016/j.bbamem.2023.184216

Boosted by the indiscriminate use of antibiotics, multidrug-resistance (MDR) demands new strategies to combat bacterial infections, such as photothermal therapy (PTT) based on plasmonic nanostructures. PTT efficiency relies on photoinduced damage caused to the bacterial machinery, for which nanostructure incorporation into the cell envelope is key. Herein, we shall unveil the binding and photochemical mechanisms of gold shell-isolated nanorods (AuSHINRs) on bioinspired bacterial membranes assembled as Langmuir and Langmuir-Schaefer (LS) monolayers of DOPE, Lysyl-PG, DOPG and CL. AuSHINRs incorporation expanded the isotherms, with stronger effect on the anionic DOPG and CL. Indeed, FTIR of LS films revealed more modifications for DOPG and CL owing to stronger attractive electrostatic interactions between anionic phosphates and the positively charged AuSHINRs, while electrostatic repulsions with the cationic ethanolamine (DOPE) and lysyl (Lysyl-PG) polar groups might have weakened their interactions with AuSHINRs. No statistical difference was observed in the surface area of irradiated DOPE and Lysyl-PG monolayers on AuSHINRs, which is evidence of the restricted nanostructures insertion. In contrast, irradiated DOPG monolayer on AuSHINRs decreased 4.0 % in surface area, while irradiated CL monolayer increased 3.7 %. Such results agree with oxidative reactions prompted by ROS generated by AuSHINRs photoactivation. The deepest AuSHINRs insertion into DOPG may have favored chain cleavage while hydroperoxidation is the mostly like outcome in CL, where AuSHINRs are surrounding the polar groups. Furthermore, preliminary experiments on Escherichia coli culture demonstrated that the electrostatic interactions with AuSHINRs do not inhibit bacterial growth, but the photoinduced effects are highly toxic, resulting in microbial inactivation. [Display omitted] •Shell-isolated gold nanorods (AuSHINRs) were applied here as photothermal agents.•Bioinspired monolayers of bacterial membranes were used as target cell models.•Electrostatic interactions may determine the binding mechanism between AuSHINRs and lipid membranes.•The photochemical reactions triggered depend on the AuSHINRs binding sites.