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Potential function of Scx+/Sox9+ cells as progenitor cells in rotator cuff tear repair in rats
Tendons and their attachment sites to bone, fibrocartilaginous tissues, have poor self-repair capacity when they rupture, and have risks of retear even after surgical repair. Thus, defining mechanisms underlying their repair is required in order to stimulate tendon repairing capacity. Here we used a...
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Published in: | Biochemical and biophysical research communications 2023-10, Vol.676, p.84-90 |
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creator | Fukuma, Yuko Tokunaga, Takuya Tanimura, Shuntaro Yoshimoto, Yuki Mashimo, Tomoji Kaneko, Takehito Tian, Xiao Ideo, Katsumasa Yonemitsu, Ryuji Matsushita, Kozo Sugimoto, Kazuki Yugami, Masaki Hisanaga, Satoshi Nakamura, Takayuki Uehara, Yusuke Masuda, Tetsuro Shukunami, Chisa Karasugi, Tatsuki Miyamoto, Takeshi |
description | Tendons and their attachment sites to bone, fibrocartilaginous tissues, have poor self-repair capacity when they rupture, and have risks of retear even after surgical repair. Thus, defining mechanisms underlying their repair is required in order to stimulate tendon repairing capacity. Here we used a rat surgical rotator cuff tear repair model and identified cells expressing the transcription factors Scleraxis (Scx) and SRY-box 9 (Sox9) as playing a crucial role in rotator cuff tendon-to-bone repair. Given the challenges of establishing stably reproducible models of surgical rotator cuff tear repair in mice, we newly established Scx-GFP transgenic rats in which Scx expression can be monitored by GFP. We observed tissue-specific GFP expression along tendons in developing ScxGFP transgenic rats and were able to successfully monitor tissue-specific Scx expression based on GFP signals. Among 3-, 6-, and 12-week-old ScxGFP rats, Scx+/Sox9+ cells were most abundant in 3-week-old rats near the site of humerus bone attachment to the rotator cuff tendon, while we observed significantly fewer cells in the same area in 6- or 12-week-old rats. We then applied a rotator cuff repair model using ScxGFP rats and observed the largest number of Scx+/Sox9+ cells at postoperative repair sites of 3-week-old relative to 6- or 12-week-old rats. Tendons attach to bone via fibrocartilaginous tissue, and cartilage-like tissue was seen at repair sites of 3-week-old but not 6- or 12-week-old rats during postoperative evaluation. Our findings suggest that Scx+/Sox9+ cells may function in rotator cuff repair, and that ScxGFP rats could serve as useful tools to develop therapies to promote rotator cuff repair by enabling analysis of these activities.
•We newly established ScxGFP transgenic rats to detect Scx expression by GFP signals.•A surgical rotator cuff tear repair model was created in ScxGFP transgenic rats.•Scx+/Sox9+ cells were most abundant at repair site in 3- than 6- or 12-week-old rats.•Fibrocartilaginous tissue was induced at repair site in only 3-week-old rats. |
doi_str_mv | 10.1016/j.bbrc.2023.07.039 |
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•We newly established ScxGFP transgenic rats to detect Scx expression by GFP signals.•A surgical rotator cuff tear repair model was created in ScxGFP transgenic rats.•Scx+/Sox9+ cells were most abundant at repair site in 3- than 6- or 12-week-old rats.•Fibrocartilaginous tissue was induced at repair site in only 3-week-old rats.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2023.07.039</identifier><identifier>PMID: 37499368</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Rotator cuff tear ; Scleraxis ; Sox9</subject><ispartof>Biochemical and biophysical research communications, 2023-10, Vol.676, p.84-90</ispartof><rights>2023 Elsevier Inc.</rights><rights>Copyright © 2023 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-785fed999f09ab8d708bea0455c74cf104d682d3130e3a84c862a598b50bf5603</citedby><cites>FETCH-LOGICAL-c444t-785fed999f09ab8d708bea0455c74cf104d682d3130e3a84c862a598b50bf5603</cites><orcidid>0000-0003-0460-2087 ; 0000-0003-1186-3760 ; 0000-0002-7052-3013</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37499368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukuma, Yuko</creatorcontrib><creatorcontrib>Tokunaga, Takuya</creatorcontrib><creatorcontrib>Tanimura, Shuntaro</creatorcontrib><creatorcontrib>Yoshimoto, Yuki</creatorcontrib><creatorcontrib>Mashimo, Tomoji</creatorcontrib><creatorcontrib>Kaneko, Takehito</creatorcontrib><creatorcontrib>Tian, Xiao</creatorcontrib><creatorcontrib>Ideo, Katsumasa</creatorcontrib><creatorcontrib>Yonemitsu, Ryuji</creatorcontrib><creatorcontrib>Matsushita, Kozo</creatorcontrib><creatorcontrib>Sugimoto, Kazuki</creatorcontrib><creatorcontrib>Yugami, Masaki</creatorcontrib><creatorcontrib>Hisanaga, Satoshi</creatorcontrib><creatorcontrib>Nakamura, Takayuki</creatorcontrib><creatorcontrib>Uehara, Yusuke</creatorcontrib><creatorcontrib>Masuda, Tetsuro</creatorcontrib><creatorcontrib>Shukunami, Chisa</creatorcontrib><creatorcontrib>Karasugi, Tatsuki</creatorcontrib><creatorcontrib>Miyamoto, Takeshi</creatorcontrib><title>Potential function of Scx+/Sox9+ cells as progenitor cells in rotator cuff tear repair in rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Tendons and their attachment sites to bone, fibrocartilaginous tissues, have poor self-repair capacity when they rupture, and have risks of retear even after surgical repair. Thus, defining mechanisms underlying their repair is required in order to stimulate tendon repairing capacity. Here we used a rat surgical rotator cuff tear repair model and identified cells expressing the transcription factors Scleraxis (Scx) and SRY-box 9 (Sox9) as playing a crucial role in rotator cuff tendon-to-bone repair. Given the challenges of establishing stably reproducible models of surgical rotator cuff tear repair in mice, we newly established Scx-GFP transgenic rats in which Scx expression can be monitored by GFP. We observed tissue-specific GFP expression along tendons in developing ScxGFP transgenic rats and were able to successfully monitor tissue-specific Scx expression based on GFP signals. Among 3-, 6-, and 12-week-old ScxGFP rats, Scx+/Sox9+ cells were most abundant in 3-week-old rats near the site of humerus bone attachment to the rotator cuff tendon, while we observed significantly fewer cells in the same area in 6- or 12-week-old rats. We then applied a rotator cuff repair model using ScxGFP rats and observed the largest number of Scx+/Sox9+ cells at postoperative repair sites of 3-week-old relative to 6- or 12-week-old rats. Tendons attach to bone via fibrocartilaginous tissue, and cartilage-like tissue was seen at repair sites of 3-week-old but not 6- or 12-week-old rats during postoperative evaluation. Our findings suggest that Scx+/Sox9+ cells may function in rotator cuff repair, and that ScxGFP rats could serve as useful tools to develop therapies to promote rotator cuff repair by enabling analysis of these activities.
•We newly established ScxGFP transgenic rats to detect Scx expression by GFP signals.•A surgical rotator cuff tear repair model was created in ScxGFP transgenic rats.•Scx+/Sox9+ cells were most abundant at repair site in 3- than 6- or 12-week-old rats.•Fibrocartilaginous tissue was induced at repair site in only 3-week-old rats.</description><subject>Rotator cuff tear</subject><subject>Scleraxis</subject><subject>Sox9</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLJDEQhYOs6Kz6BzwsOS5It5V0ujsBLyK6LggKKngypNMVyTDTmU3Sov_eHmfco6eCV68erz5CjhmUDFhzOi-7LtqSA69KaEuo1A6ZMVBQcAbiB5kBQFNwxZ72yc-U5gCMiUbtkf2qFUpVjZyR57uQccjeLKgbB5t9GGhw9N6-nZzehzd1Qi0uFomaRFcxvODgc4hbzQ80hmw-hdE5mtFEGnFlfPzcmZwOya4zi4RH23lAHq8uHy6ui5vbP38vzm8KK4TIRStrh71SyoEynexbkB0aEHVtW2Hd9E3fSN5XrAKsjBRWNtzUSnY1dK5uoDogvze5U8l_I6aslz6tW5oBw5g0l7UQXCjBJivfWG0MKUV0ehX90sR3zUCvueq5XnPVa64aWj1xnY5-bfPHbon9_5MvkJPhbGPA6ctXj1En63Gw2PuINus--O_yPwD3A4jZ</recordid><startdate>20231008</startdate><enddate>20231008</enddate><creator>Fukuma, Yuko</creator><creator>Tokunaga, Takuya</creator><creator>Tanimura, Shuntaro</creator><creator>Yoshimoto, Yuki</creator><creator>Mashimo, Tomoji</creator><creator>Kaneko, Takehito</creator><creator>Tian, Xiao</creator><creator>Ideo, Katsumasa</creator><creator>Yonemitsu, Ryuji</creator><creator>Matsushita, Kozo</creator><creator>Sugimoto, Kazuki</creator><creator>Yugami, Masaki</creator><creator>Hisanaga, Satoshi</creator><creator>Nakamura, Takayuki</creator><creator>Uehara, Yusuke</creator><creator>Masuda, Tetsuro</creator><creator>Shukunami, Chisa</creator><creator>Karasugi, Tatsuki</creator><creator>Miyamoto, Takeshi</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0460-2087</orcidid><orcidid>https://orcid.org/0000-0003-1186-3760</orcidid><orcidid>https://orcid.org/0000-0002-7052-3013</orcidid></search><sort><creationdate>20231008</creationdate><title>Potential function of Scx+/Sox9+ cells as progenitor cells in rotator cuff tear repair in rats</title><author>Fukuma, Yuko ; 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Thus, defining mechanisms underlying their repair is required in order to stimulate tendon repairing capacity. Here we used a rat surgical rotator cuff tear repair model and identified cells expressing the transcription factors Scleraxis (Scx) and SRY-box 9 (Sox9) as playing a crucial role in rotator cuff tendon-to-bone repair. Given the challenges of establishing stably reproducible models of surgical rotator cuff tear repair in mice, we newly established Scx-GFP transgenic rats in which Scx expression can be monitored by GFP. We observed tissue-specific GFP expression along tendons in developing ScxGFP transgenic rats and were able to successfully monitor tissue-specific Scx expression based on GFP signals. Among 3-, 6-, and 12-week-old ScxGFP rats, Scx+/Sox9+ cells were most abundant in 3-week-old rats near the site of humerus bone attachment to the rotator cuff tendon, while we observed significantly fewer cells in the same area in 6- or 12-week-old rats. We then applied a rotator cuff repair model using ScxGFP rats and observed the largest number of Scx+/Sox9+ cells at postoperative repair sites of 3-week-old relative to 6- or 12-week-old rats. Tendons attach to bone via fibrocartilaginous tissue, and cartilage-like tissue was seen at repair sites of 3-week-old but not 6- or 12-week-old rats during postoperative evaluation. Our findings suggest that Scx+/Sox9+ cells may function in rotator cuff repair, and that ScxGFP rats could serve as useful tools to develop therapies to promote rotator cuff repair by enabling analysis of these activities.
•We newly established ScxGFP transgenic rats to detect Scx expression by GFP signals.•A surgical rotator cuff tear repair model was created in ScxGFP transgenic rats.•Scx+/Sox9+ cells were most abundant at repair site in 3- than 6- or 12-week-old rats.•Fibrocartilaginous tissue was induced at repair site in only 3-week-old rats.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>37499368</pmid><doi>10.1016/j.bbrc.2023.07.039</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0460-2087</orcidid><orcidid>https://orcid.org/0000-0003-1186-3760</orcidid><orcidid>https://orcid.org/0000-0002-7052-3013</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Rotator cuff tear Scleraxis Sox9 |
title | Potential function of Scx+/Sox9+ cells as progenitor cells in rotator cuff tear repair in rats |
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