Probiotics enhance resistance to Streptococcus iniae in Nile tilapia (Oreochromis niloticus) reared in biofloc systems

Biofloc technology is a rearing technique that maintains desired water quality by manipulating carbon and nitrogen and their inherent mixture of organic matter and microbes. Beneficial microorganisms in biofloc systems produce bioactive metabolites that may deter the growth of pathogenic microbes. A...

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Bibliographic Details
Published in:Journal of fish diseases 2023-10, Vol.46 (10), p.1137-1149
Main Authors: Padeniya, Uthpala, Davis, D Allen, Liles, Mark R, LaFrentz, Stacey A, LaFrentz, Benjamin R, Shoemaker, Craig A, Beck, Benjamin H, Wells, Daniel E, Bruce, Timothy J
Format: Article
Language:English
Subjects:
Bacteria
Bioactive compounds
Biofloc technology
Design of experiments
Diet
Dietary supplements
Experimental design
Fish
Freshwater fishes
Gene expression
Juveniles
Lysozyme
Marine fishes
Metabolites
Microorganisms
Oreochromis niloticus
Organic matter
Pathogens
Probiotics
Spleen
Streptococcus iniae
Tanks
Tilapia
Water quality
Whitefish
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Summary:Biofloc technology is a rearing technique that maintains desired water quality by manipulating carbon and nitrogen and their inherent mixture of organic matter and microbes. Beneficial microorganisms in biofloc systems produce bioactive metabolites that may deter the growth of pathogenic microbes. As little is known about the interaction of biofloc systems and the addition of probiotics, this study focused on this integration to manipulate the microbial community and its interactions within biofloc systems. The present study evaluated two probiotics (B. velezensis AP193 and BiOWiSH FeedBuilder Syn 3) for use in Nile tilapia (Oreochromis niloticus) culture in a biofloc system. Nine independent 3785 L circular tanks were stocked with 120 juveniles (71.4 ± 4.4 g). Tilapia were fed for 16 weeks and randomly assigned three diets: a commercial control diet or a commercial diet top-coated with either AP193 or BiOWiSH FeedBuilder Syn3. At 14 weeks, the fish were challenged with a low dose of Streptococcus iniae (ARS-98-60, 7.2 × 10  CFU mL , via intraperitoneal injection) in a common garden experimental design. At 16 weeks, the fish were challenged with a high dose of S. iniae (6.6 × 10  CFU mL ) in the same manner. At the end of each challenge trial, cumulative per cent mortality, lysozyme activity and expression of 4 genes (il-1β, il6, il8 and tnfα) from the spleen were measured. In both challenges, the mortalities of the probiotic-fed groups were significantly lower (p 
ISSN:0140-7775
1365-2761
DOI:10.1111/jfd.13833