A draft reference genome assembly of the Pipevine Swallowtail butterfly, Battus philenor hirsuta

Abstract The California Pipevine Swallowtail Butterfly, Battus philenor hirsuta, and its host plant, the California Pipevine or Dutchman’s Pipe, Aristolochia californica Torr., are an important California endemic species pair. While this species pair is an ideal system to study co-evolution, genomic...

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Bibliographic Details
Published in:The Journal of heredity 2023-11, Vol.114 (6), p.698-706
Main Authors: Chaturvedi, Samridhi, Escalona, Merly, Marimuthu, Mohan P A, Nguyen, Oanh, Chumchim, Noravit, Fairbairn, Colin W, Seligmann, William, Miller, Courtney, Bradley Shaffer, H, Whiteman, Noah K
Format: Article
Language:English
Subjects:
Animals
Aristolochia - genetics
Butterflies - genetics
Chromosomes
Genome
Genomics
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Summary:Abstract The California Pipevine Swallowtail Butterfly, Battus philenor hirsuta, and its host plant, the California Pipevine or Dutchman’s Pipe, Aristolochia californica Torr., are an important California endemic species pair. While this species pair is an ideal system to study co-evolution, genomic resources for both are lacking. Here, we report a new, chromosome-level assembly of B. philenor hirsuta as part of the California Conservation Genomics Project (CCGP). Following the sequencing and assembly strategy of the CCGP, we used Pacific Biosciences HiFi long reads and Hi-C chromatin proximity sequencing technology to produce a de novo assembled genome. Our genome assembly, the first for any species in the genus, contains 109 scaffolds spanning 443 mega base (Mb) pairs, with a contig N50 of 14.6 Mb, a scaffold N50 of 15.2 Mb, and BUSCO complete score of 98.9%. In combination with the forthcoming A. californica reference genome, the B. philenor hirsuta genome will be a powerful tool for documenting landscape genomic diversity and plant–insect co-evolution in a rapidly changing California landscape.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/esad043