Tracking new insights into antifungal and anti-mycotoxigenic properties of a biofilm forming Pediococcus pentosaceus strain isolated from grain silage

• Published in: International journal of food microbiology 2023-11, Vol.405, p.110337-110337, Article 110337
• Main Authors: Mendonça, Carlos M.N., Oliveira, Rodrigo C., Pizauro, Lucas J.L., Pereira, Wellison A., Abboud, Kahlile, Almeida, Sonia, Watanabe, Ii-Sei, Varani, Alessandro M., Domínguez, José M., Correa, Benedito, Venema, Koen, Azevedo, Pamela O.S., Oliveira, Ricardo P.S.
• Format: Article
• Language: English
• Subjects: Aflatoxins; Biofilm; Crops contamination; Fumonisin; Lactic acid bacteria; Mycotoxin
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2023.110337

The present study offers detailed insights into the antifungal and anti-mycotoxigenic potential of a biofilm forming lactic acid bacterium (Pediococcus pentosaceus) against one atoxigenic (Aspergillus flavus) and two toxigenic (Aspergillus nomius and Fusarium verticillioides) fungal strains. The antifungal effect of P. pentosaceus LBM18 strain was initially investigated through comparative analysis of fungi physiology by macroscopic visual evaluations and scanning electron microscopy examinations. The effects over fungal growth rate and asexual sporulation were additionally accessed. Furthermore, analytical evaluations of mycotoxin production were carried out by HPLC-MS/MS to provide insights on the bacterial anti-mycotoxigenic activity over fungal production of the aflatoxins B1, B2, G1 and G2 as well as fumonisins B1 and B2. Finally, reverse transcription quantitative real-time PCR (RT-qPCR) analysis was employed at the most effective bacterial inoculant concentration to evaluate, at the molecular level, the down-regulation of genes aflR, aflQ and aflD, related to the biosynthesis of aflatoxins by the strain of Aspergillus nomius. The effects over mycotoxin contamination were thought to be result of a combination of several biotic and abiotic factors, such as interaction between living beings and physical-chemical aspects of the environment, respectively. Several possible mechanisms of action were addressed along with potentially deleterious effects ascribing from P. pentosaceus misuse as biopesticide, emphasizing the importance of evaluating lactic acid bacteria safety in new applications, concentrations, and exposure scenarios. •Pediococcus pentosaceus presents potentially deleterious effects on mycotoxins•Promising use of Pediococcus pentosaceus as a biopesticide•Antifungal effect of Pediococcus pentosaceus•Morphological changes of atoxigenic and toxigenic fungi by Pediococcus pentosaceus