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Production of yellow‐flowered gentian plants by genetic engineering of betaxanthin pigments
Summary Genetic engineering of flower color provides biotechnological products such as blue carnations or roses by accumulating delphinidin‐based anthocyanins not naturally existing in these plant species. Betalains are another class of pigments that in plants are only synthesized in the order Caryo...
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| Published in: | The New phytologist 2023-11, Vol.240 (3), p.1177-1188 |
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| Main Authors: | , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c3308-157d5b6c6e5fb510bee2eec2a6380347efe798f476a8f0cefc9c9e2ab8b7349d3 |
| container_end_page | 1188 |
| container_issue | 3 |
| container_start_page | 1177 |
| container_title | The New phytologist |
| container_volume | 240 |
| creator | Nishihara, Masahiro Hirabuchi, Akiko Goto, Fumina Nishizaki, Yuzo Uesugi, Shota Watanabe, Aiko Tasaki, Keisuke Washiashi, Rie Sasaki, Nobuhiro |
| description | Summary
Genetic engineering of flower color provides biotechnological products such as blue carnations or roses by accumulating delphinidin‐based anthocyanins not naturally existing in these plant species. Betalains are another class of pigments that in plants are only synthesized in the order Caryophyllales. Although they have been engineered in several plant species, especially red‐violet betacyanins, the yellow betaxanthins have yet to be engineered in ornamental plants.
We attempted to produce yellow‐flowered gentians by genetic engineering of betaxanthin pigments. First, white‐flowered gentian lines were produced by knocking out the dihydroflavonol 4‐reductase (DFR) gene using CRISPR/Cas9‐mediated genome editing.
Beta vulgaris BvCYP76AD6 and Mirabilis jalapa MjDOD, driven by gentian petal‐specific promoters, flavonoid 3′,5′‐hydroxylase (F3′5′H) and anthocyanin 5,3′‐aromatic acyltransferase (AT), respectively, were transformed into the above DFR‐knockout white‐flowered line; the resultant gentian plants had vivid yellow flowers. Expression analysis and pigment analysis revealed petal‐specific expression and accumulation of seven known betaxanthins in their petals to c. 0.06–0.08 μmol g FW−1.
Genetic engineering of vivid yellow‐flowered plants can be achieved by combining genome editing and a suitable expression of betaxanthin‐biosynthetic genes in ornamental plants. |
| doi_str_mv | 10.1111/nph.19218 |
| format | article |
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Genetic engineering of flower color provides biotechnological products such as blue carnations or roses by accumulating delphinidin‐based anthocyanins not naturally existing in these plant species. Betalains are another class of pigments that in plants are only synthesized in the order Caryophyllales. Although they have been engineered in several plant species, especially red‐violet betacyanins, the yellow betaxanthins have yet to be engineered in ornamental plants.
We attempted to produce yellow‐flowered gentians by genetic engineering of betaxanthin pigments. First, white‐flowered gentian lines were produced by knocking out the dihydroflavonol 4‐reductase (DFR) gene using CRISPR/Cas9‐mediated genome editing.
Beta vulgaris BvCYP76AD6 and Mirabilis jalapa MjDOD, driven by gentian petal‐specific promoters, flavonoid 3′,5′‐hydroxylase (F3′5′H) and anthocyanin 5,3′‐aromatic acyltransferase (AT), respectively, were transformed into the above DFR‐knockout white‐flowered line; the resultant gentian plants had vivid yellow flowers. Expression analysis and pigment analysis revealed petal‐specific expression and accumulation of seven known betaxanthins in their petals to c. 0.06–0.08 μmol g FW−1.
Genetic engineering of vivid yellow‐flowered plants can be achieved by combining genome editing and a suitable expression of betaxanthin‐biosynthetic genes in ornamental plants.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.19218</identifier><language>eng</language><publisher>Lancaster: Wiley Subscription Services, Inc</publisher><subject>Accumulation ; Acyltransferase ; Anthocyanins ; Aromatic compounds ; betalain ; betaxanthin ; Colour ; CRISPR ; CRISPR/Cas9 ; DFR ; Editing ; Flavonoids ; flower color ; Flowers ; Flowers & plants ; Gene editing ; Gene expression ; Genetic engineering ; genome editing ; Genomes ; Japanese gentian ; Ornamental plants ; Petals ; Pigments ; Plant species ; Plants ; Plants (botany) ; Reductases ; vivid yellow color</subject><ispartof>The New phytologist, 2023-11, Vol.240 (3), p.1177-1188</ispartof><rights>2023 The Authors. © 2023 New Phytologist Foundation</rights><rights>Copyright © 2023 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3308-157d5b6c6e5fb510bee2eec2a6380347efe798f476a8f0cefc9c9e2ab8b7349d3</citedby><cites>FETCH-LOGICAL-c3308-157d5b6c6e5fb510bee2eec2a6380347efe798f476a8f0cefc9c9e2ab8b7349d3</cites><orcidid>0000-0001-6470-7804 ; 0000-0001-5640-9567 ; 0000-0001-7081-2176 ; 0000-0002-1454-8785 ; 0000-0001-7474-9493 ; 0000-0003-3547-5746</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Nishihara, Masahiro</creatorcontrib><creatorcontrib>Hirabuchi, Akiko</creatorcontrib><creatorcontrib>Goto, Fumina</creatorcontrib><creatorcontrib>Nishizaki, Yuzo</creatorcontrib><creatorcontrib>Uesugi, Shota</creatorcontrib><creatorcontrib>Watanabe, Aiko</creatorcontrib><creatorcontrib>Tasaki, Keisuke</creatorcontrib><creatorcontrib>Washiashi, Rie</creatorcontrib><creatorcontrib>Sasaki, Nobuhiro</creatorcontrib><title>Production of yellow‐flowered gentian plants by genetic engineering of betaxanthin pigments</title><title>The New phytologist</title><description>Summary
Genetic engineering of flower color provides biotechnological products such as blue carnations or roses by accumulating delphinidin‐based anthocyanins not naturally existing in these plant species. Betalains are another class of pigments that in plants are only synthesized in the order Caryophyllales. Although they have been engineered in several plant species, especially red‐violet betacyanins, the yellow betaxanthins have yet to be engineered in ornamental plants.
We attempted to produce yellow‐flowered gentians by genetic engineering of betaxanthin pigments. First, white‐flowered gentian lines were produced by knocking out the dihydroflavonol 4‐reductase (DFR) gene using CRISPR/Cas9‐mediated genome editing.
Beta vulgaris BvCYP76AD6 and Mirabilis jalapa MjDOD, driven by gentian petal‐specific promoters, flavonoid 3′,5′‐hydroxylase (F3′5′H) and anthocyanin 5,3′‐aromatic acyltransferase (AT), respectively, were transformed into the above DFR‐knockout white‐flowered line; the resultant gentian plants had vivid yellow flowers. Expression analysis and pigment analysis revealed petal‐specific expression and accumulation of seven known betaxanthins in their petals to c. 0.06–0.08 μmol g FW−1.
Genetic engineering of vivid yellow‐flowered plants can be achieved by combining genome editing and a suitable expression of betaxanthin‐biosynthetic genes in ornamental plants.</description><subject>Accumulation</subject><subject>Acyltransferase</subject><subject>Anthocyanins</subject><subject>Aromatic compounds</subject><subject>betalain</subject><subject>betaxanthin</subject><subject>Colour</subject><subject>CRISPR</subject><subject>CRISPR/Cas9</subject><subject>DFR</subject><subject>Editing</subject><subject>Flavonoids</subject><subject>flower color</subject><subject>Flowers</subject><subject>Flowers & plants</subject><subject>Gene editing</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>genome editing</subject><subject>Genomes</subject><subject>Japanese gentian</subject><subject>Ornamental plants</subject><subject>Petals</subject><subject>Pigments</subject><subject>Plant species</subject><subject>Plants</subject><subject>Plants (botany)</subject><subject>Reductases</subject><subject>vivid yellow color</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp10MFKwzAYB_AgCs7pwTcoeNFDtyRt0_QoQ50wdAcFLxLS7EuX0aUzaZm7-Qg-o09iZj0J5vJB-P0_kj9C5wSPSDhju1mOSEEJP0ADkrIi5iTJD9EAY8pjlrKXY3Ti_QpjXGSMDtDr3DWLTrWmsVGjox3UdbP9-vjUYYCDRVSBbY200aaWtvVRudvfQGtUBLYyFsAZW-2jJbTyPZilCdhU65Dzp-hIy9rD2e8coufbm6fJNJ493t1PrmexShLMY5Lli6xkikGmy4zgEoACKCpZwnGS5qAhL7hOcya5xgq0KlQBVJa8zJO0WCRDdNnv3bjmrQPfirXxKvxFWmg6LyjP0oLtdwV68Yeums7Z8LqgcprxPKUkqKteKdd470CLjTNr6XaCYLEvWoSixU_RwY57uzU17P6H4mE-7RPfMTmCMg</recordid><startdate>202311</startdate><enddate>202311</enddate><creator>Nishihara, Masahiro</creator><creator>Hirabuchi, Akiko</creator><creator>Goto, Fumina</creator><creator>Nishizaki, Yuzo</creator><creator>Uesugi, Shota</creator><creator>Watanabe, Aiko</creator><creator>Tasaki, Keisuke</creator><creator>Washiashi, Rie</creator><creator>Sasaki, Nobuhiro</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6470-7804</orcidid><orcidid>https://orcid.org/0000-0001-5640-9567</orcidid><orcidid>https://orcid.org/0000-0001-7081-2176</orcidid><orcidid>https://orcid.org/0000-0002-1454-8785</orcidid><orcidid>https://orcid.org/0000-0001-7474-9493</orcidid><orcidid>https://orcid.org/0000-0003-3547-5746</orcidid></search><sort><creationdate>202311</creationdate><title>Production of yellow‐flowered gentian plants by genetic engineering of betaxanthin pigments</title><author>Nishihara, Masahiro ; Hirabuchi, Akiko ; Goto, Fumina ; Nishizaki, Yuzo ; Uesugi, Shota ; Watanabe, Aiko ; Tasaki, Keisuke ; Washiashi, Rie ; Sasaki, Nobuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3308-157d5b6c6e5fb510bee2eec2a6380347efe798f476a8f0cefc9c9e2ab8b7349d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Accumulation</topic><topic>Acyltransferase</topic><topic>Anthocyanins</topic><topic>Aromatic compounds</topic><topic>betalain</topic><topic>betaxanthin</topic><topic>Colour</topic><topic>CRISPR</topic><topic>CRISPR/Cas9</topic><topic>DFR</topic><topic>Editing</topic><topic>Flavonoids</topic><topic>flower color</topic><topic>Flowers</topic><topic>Flowers & plants</topic><topic>Gene editing</topic><topic>Gene expression</topic><topic>Genetic engineering</topic><topic>genome editing</topic><topic>Genomes</topic><topic>Japanese gentian</topic><topic>Ornamental plants</topic><topic>Petals</topic><topic>Pigments</topic><topic>Plant species</topic><topic>Plants</topic><topic>Plants (botany)</topic><topic>Reductases</topic><topic>vivid yellow color</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishihara, Masahiro</creatorcontrib><creatorcontrib>Hirabuchi, Akiko</creatorcontrib><creatorcontrib>Goto, Fumina</creatorcontrib><creatorcontrib>Nishizaki, Yuzo</creatorcontrib><creatorcontrib>Uesugi, Shota</creatorcontrib><creatorcontrib>Watanabe, Aiko</creatorcontrib><creatorcontrib>Tasaki, Keisuke</creatorcontrib><creatorcontrib>Washiashi, Rie</creatorcontrib><creatorcontrib>Sasaki, Nobuhiro</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishihara, Masahiro</au><au>Hirabuchi, Akiko</au><au>Goto, Fumina</au><au>Nishizaki, Yuzo</au><au>Uesugi, Shota</au><au>Watanabe, Aiko</au><au>Tasaki, Keisuke</au><au>Washiashi, Rie</au><au>Sasaki, Nobuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of yellow‐flowered gentian plants by genetic engineering of betaxanthin pigments</atitle><jtitle>The New phytologist</jtitle><date>2023-11</date><risdate>2023</risdate><volume>240</volume><issue>3</issue><spage>1177</spage><epage>1188</epage><pages>1177-1188</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
Genetic engineering of flower color provides biotechnological products such as blue carnations or roses by accumulating delphinidin‐based anthocyanins not naturally existing in these plant species. Betalains are another class of pigments that in plants are only synthesized in the order Caryophyllales. Although they have been engineered in several plant species, especially red‐violet betacyanins, the yellow betaxanthins have yet to be engineered in ornamental plants.
We attempted to produce yellow‐flowered gentians by genetic engineering of betaxanthin pigments. First, white‐flowered gentian lines were produced by knocking out the dihydroflavonol 4‐reductase (DFR) gene using CRISPR/Cas9‐mediated genome editing.
Beta vulgaris BvCYP76AD6 and Mirabilis jalapa MjDOD, driven by gentian petal‐specific promoters, flavonoid 3′,5′‐hydroxylase (F3′5′H) and anthocyanin 5,3′‐aromatic acyltransferase (AT), respectively, were transformed into the above DFR‐knockout white‐flowered line; the resultant gentian plants had vivid yellow flowers. Expression analysis and pigment analysis revealed petal‐specific expression and accumulation of seven known betaxanthins in their petals to c. 0.06–0.08 μmol g FW−1.
Genetic engineering of vivid yellow‐flowered plants can be achieved by combining genome editing and a suitable expression of betaxanthin‐biosynthetic genes in ornamental plants.</abstract><cop>Lancaster</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/nph.19218</doi><tpages>1188</tpages><orcidid>https://orcid.org/0000-0001-6470-7804</orcidid><orcidid>https://orcid.org/0000-0001-5640-9567</orcidid><orcidid>https://orcid.org/0000-0001-7081-2176</orcidid><orcidid>https://orcid.org/0000-0002-1454-8785</orcidid><orcidid>https://orcid.org/0000-0001-7474-9493</orcidid><orcidid>https://orcid.org/0000-0003-3547-5746</orcidid></addata></record> |
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| ispartof | The New phytologist, 2023-11, Vol.240 (3), p.1177-1188 |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Accumulation Acyltransferase Anthocyanins Aromatic compounds betalain betaxanthin Colour CRISPR CRISPR/Cas9 DFR Editing Flavonoids flower color Flowers Flowers & plants Gene editing Gene expression Genetic engineering genome editing Genomes Japanese gentian Ornamental plants Petals Pigments Plant species Plants Plants (botany) Reductases vivid yellow color |
| title | Production of yellow‐flowered gentian plants by genetic engineering of betaxanthin pigments |
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