A colorimetric analytical method based on a TCPP–CuCo2O4-like peroxidase for the detection of trichlorfon

In this work, a highly sensitive colorimetric sensing platform was designed for the detection of trichlorfon based on inhibiting thiocholine (TCh)-induced redox reaction. 5,10,15,20-Tetracarboxyphenylporphyrin (TCPP) functionalized CuCo2O4 (TCPP–CuCo2O4) was synthesized to construct a colorimetric s...

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Bibliographic Details
Published in:Analytical methods 2023-08, Vol.15 (34), p.4331-4337
Main Authors: Xiao, Xin, Liao, Wenchun, Rao, Ma, Huang, Long, Yang, Yunhui
Format: Article
Language:English
Subjects:
Absorption
Acetylcholinesterase
Colorimetry
Correlation coefficient
Correlation coefficients
Hydrogen peroxide
Organophosphorus pesticides
Peroxidase
Redox reactions
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Summary:In this work, a highly sensitive colorimetric sensing platform was designed for the detection of trichlorfon based on inhibiting thiocholine (TCh)-induced redox reaction. 5,10,15,20-Tetracarboxyphenylporphyrin (TCPP) functionalized CuCo2O4 (TCPP–CuCo2O4) was synthesized to construct a colorimetric sensing platform for trichlorfon. In the presence of H2O2, TCPP–CuCo2O4 can oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue ox-TMB, accompanied by a strong absorption peak at 652 nm, while acetylcholinesterase (AChE) can specifically hydrolyze acetylthiocholine (ATCh) into TCh, which can reduce ox-TMB back into colorless TMB, resulting in a lower absorbance at 652 nm. Trichlorfon can irreversibly inhibit the activity of AChE and thus recover the absorption peak. Under the optimized conditions, detection of trichlorfon has a wide linear range of 40–4000 ng mL−1 with a linear correlation coefficient of 0.9904. The proposed method can be applied to the detection of trichlorfon in vegetables and has good application prospects.
ISSN:1759-9660
1759-9679
DOI:10.1039/d3ay01057k