Emission Wavelength-Tunable Bicyclic Dioxetane Chemiluminescent Probes for Precise In Vitro and In Vivo Imaging

Chemiluminescent probes have become increasingly popular in various research areas including precise tumor imaging and immunofluorescence analysis. Nevertheless, previously developed chemiluminescence probes are mainly limited to studying oxidation reaction-associated biological events. This study p...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-09, Vol.95 (35), p.13191-13200
Main Authors: Li, Shen-Huan, Zhang, Guo-Rong, He, Yu-Ting, Yang, Liu, Li, Han-Lu, Long, Cheng-Yu, Cui, Yue, Wang, Xue-Qiang
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemiluminescence
Chemistry
Emission
Emissions
Hepatocytes
Hydrogen peroxide
Imaging techniques
Immunofluorescence
Liver cancer
Macrophages
Medical imaging
Ovarian cancer
Oxidation
Probes
Superoxide
Tumors
Wavelengths
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chemiluminescent probes have become increasingly popular in various research areas including precise tumor imaging and immunofluorescence analysis. Nevertheless, previously developed chemiluminescence probes are mainly limited to studying oxidation reaction-associated biological events. This study presents the first example of bioimaging applicable bicyclic dioxetane chemiluminescent probes with tunable emission wavelengths that range from 525 to 800 nm. These newly developed probes were able to detect the analytes of β-Gal, H2O2, and superoxide with high specificity and a limit of detection of 77 mU L–1, 96, and 28 nM, respectively. The bioimaging application of the probes was verified in ovarian and liver cancer cells and macrophage cells, allowing the detection of the content of β-Gal, H2O2, and superoxide inside the cells. The high specificity allowed us to image the xenografted tumor in mice. We expect that our probes will receive extensive applications in recording complex biomolecular events using noninvasive imaging techniques.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c02126