Thermal Adaptation Alters Response to Thermal Stress and Expression of Virulent Genes (eae, stx1, stx2, and hlyA) in Pathogenic Escherichia coli Isolated from Pork

Escherichia coli encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic E. coli were evaluated. Among 176 E....

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Bibliographic Details
Published in:Current microbiology 2023-10, Vol.80 (10), p.330-330, Article 330
Main Authors: Singha, Songeeta, Thomas, Rajendran, Vishwakarma, Jai N., Bharadwaj, Devarshi, Gupta, Vivek K.
Format: Article
Language:English
Subjects:
Adaptation
adhesion
Biomedical and Life Sciences
Biotechnology
E coli
Escherichia coli
Gene expression
Genes
heat tolerance
hemolysis
High temperature
Life Sciences
Low temperature
Microbiology
Pathogenicity
Pathogens
pathotypes
pork
Shiga toxin
temperature
Temperature tolerance
Thermal stress
Toxins
Transcription
Virulence
Virulence factors
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Summary:Escherichia coli encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic E. coli were evaluated. Among 176 E. coli isolates, 4 isolates (2.27%) were confirmed to be pathogenic E. coli, out of which 2 isolates were positive for EHEC and 2 were positive for EPEC based on their virulence factors. Thermotolerance was induced under thermal adaptation at higher temperature, regardless of the pathotypes. Cells grown and adapted at 42 °C, exhibited highest transcription of genes associated with adhesion ( eae ), hemolysis ( hly A), and shiga toxin production ( stx 1) . However, expression of these genes was downregulated in cells adapted at lower temperature of 4 °C and 25 °C compared to control. Further, transcription of stx2 was upregulated by 70% and 17% at 4 °C and 25 °C, respectively, while the transcription level was reduced by 44% relative to control at 42 °C. The findings indicate that expression of virulent genes in pathogenic E. coli at elevated temperature do not be depend on thermotolerance of the strain harboring these genes.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-023-03446-2